摘要
运用454焦磷酸测序技术分析了健康虾夷扇贝和缺刻症状虾夷扇贝外套膜细菌多样性,分别从健康和缺刻虾夷扇贝样品中获得20872和16333条有效序列.结果表明:缺刻虾夷扇贝样品菌群丰度和多样性分别高于健康虾夷扇贝样品;两个样品中细菌可以分为8个门,即变形菌门、厚壁菌门、放线菌门、拟杆菌门、蓝细菌门、浮霉菌门、螺旋体门和柔膜菌门,其中前7个门类的细菌在健康和缺刻虾夷扇贝样品中均有分布;在健康虾夷扇贝样品中,变形菌门占绝对优势,占整个菌群的97.7%,次优势类群厚壁菌门占0.8%;缺刻虾夷扇贝样品中,优势类群为厚壁菌门,占整个菌群的52.2%,次优势类群变形菌门占47.7%.
Pyrosequencing-technique was used to analyze and compare bacterial diversity in the mantle of healthy and incised symptoms of Patinopecten yessoensis, respectively. For the two samples, 20872 and 16333 effective sequences were selected and utilized to perform bacterial diversity analysis, respectively. Bacterial diversity and richness in the incised samples were higher than that in the healthy samples. The effective sequences acquired from both samples included eight known phyla bacteria, i. e. , Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, Cyanobacteria, Planctomycetes, Spirochaetes and Tenericutes, among which the front seven phyla were present in the two samples. Proteobacteria was absolutely dominant in the mantle of healthy P. yessoensis accounting for 97.7% of the entire bacterial community, and Firmicutes was the sub-dominant group accounting for 0.8%. In contrast, the most abundant bacterial group in the mantle of incised P. yessoensis was Firmicutes, accounting for 52.2% of the entire bacterial community, and the subdominant was Proteobacteria accounting for 47.7 %.
出处
《应用生态学报》
CAS
CSCD
北大核心
2014年第11期3344-3348,共5页
Chinese Journal of Applied Ecology
基金
国家海洋局项目(201105007-2)
辽宁省教育厅优秀人才支持计划项目(LJQ2011073)
国家星火计划项目(2012GA651002)
辽宁省教育厅项目(L2012263)资助
关键词
优势类群
变形菌门
厚壁菌门
abundant bacterial groups
Proteobacteria
Firmicutes.