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基于454焦磷酸测序分析虾夷扇贝外套膜菌群多样性 被引量:10

Bacterial diversity in the mantle of Patinopecten yessoensis revealed by 454 pyrosequencing
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摘要 运用454焦磷酸测序技术分析了健康虾夷扇贝和缺刻症状虾夷扇贝外套膜细菌多样性,分别从健康和缺刻虾夷扇贝样品中获得20872和16333条有效序列.结果表明:缺刻虾夷扇贝样品菌群丰度和多样性分别高于健康虾夷扇贝样品;两个样品中细菌可以分为8个门,即变形菌门、厚壁菌门、放线菌门、拟杆菌门、蓝细菌门、浮霉菌门、螺旋体门和柔膜菌门,其中前7个门类的细菌在健康和缺刻虾夷扇贝样品中均有分布;在健康虾夷扇贝样品中,变形菌门占绝对优势,占整个菌群的97.7%,次优势类群厚壁菌门占0.8%;缺刻虾夷扇贝样品中,优势类群为厚壁菌门,占整个菌群的52.2%,次优势类群变形菌门占47.7%. Pyrosequencing-technique was used to analyze and compare bacterial diversity in the mantle of healthy and incised symptoms of Patinopecten yessoensis, respectively. For the two samples, 20872 and 16333 effective sequences were selected and utilized to perform bacterial diversity analysis, respectively. Bacterial diversity and richness in the incised samples were higher than that in the healthy samples. The effective sequences acquired from both samples included eight known phyla bacteria, i. e. , Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, Cyanobacteria, Planctomycetes, Spirochaetes and Tenericutes, among which the front seven phyla were present in the two samples. Proteobacteria was absolutely dominant in the mantle of healthy P. yessoensis accounting for 97.7% of the entire bacterial community, and Firmicutes was the sub-dominant group accounting for 0.8%. In contrast, the most abundant bacterial group in the mantle of incised P. yessoensis was Firmicutes, accounting for 52.2% of the entire bacterial community, and the subdominant was Proteobacteria accounting for 47.7 %.
出处 《应用生态学报》 CAS CSCD 北大核心 2014年第11期3344-3348,共5页 Chinese Journal of Applied Ecology
基金 国家海洋局项目(201105007-2) 辽宁省教育厅优秀人才支持计划项目(LJQ2011073) 国家星火计划项目(2012GA651002) 辽宁省教育厅项目(L2012263)资助
关键词 优势类群 变形菌门 厚壁菌门 abundant bacterial groups Proteobacteria Firmicutes.
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  • 1Hugenholtz P, Hooper SD, Kyrpides NC. Focus: Syner-gistetes. Environmental Microbiology, 2009, 11: 1327-1329. 被引量:1
  • 2Ferguson RL, Buckley EN, Palumbo AV. Response of marine bacterioplankton to differential filtration and confinemen. Applied and Environmental Microbiology, 1984, 47: 49-55. 被引量:1
  • 3Markle JG, Frank DN, Mortin-Toth S, et al. Sex differ-ences in the gut microbiome drive hormone-dependent regulation of autoimmunity. Science, 2013, 339: 1084-1088. 被引量:1
  • 4Oikonomou G, Machado VS, Santisteban C, et al. Microbial diversity of bovine mastitic milk as described by pyrosequencing of metagenomic 16S rDNA. PLoS One, 2012, 7(10): e47671. 被引量:1
  • 5Gosalbes MJ, Durban A, Pignatelli M, et al. Metatran-scriptomic approach to analyze the functional human gut microbiota. PloS One, 2011, 6(3): e17447. 被引量:1
  • 6Margulies M, Egholm M, Altman WE, et al. Genome sequencing in microfabricated high-density picolitre reators. Nature, 2005, 437: 376-380. 被引量:1
  • 7Xia W, Zhang C, Zeng X, et al. Autotrophic growth of nitrifying community in an agricultural soil. ISME Jour-nal, 2011, 5: 1226-1236. 被引量:1
  • 8Chu H, Fierer N, Lauber CL, et al. Soil bacterial diversity in the Arctic is not fundamentally different from that found in other biomes. Environmental Microbiology, 2010, 12: 2998-3006. 被引量:1
  • 9Herlemann DP, Labrenz M, Jurgens K, et al. Transi-tions in bacterial communities along the 2000 km salinity gradient of the Baltic Sea. ISME Journal, 2011, 5: 1571-1579. 被引量:1
  • 10Bowman JS, Rasmussen S, Blom N, et al. Microbial community structure of Arctic multiyear sea ice and sur-face seawater by 454 sequencing of the 16S RNA gene. ISME Journal, 2012, 6: 11-20. 被引量:1

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