摘要
荧光相关光谱(fluorescence correlation spectroscopy,FCS)是一种单分子荧光检测技术,能够灵敏地检测荧光分子的浓度、运动参数及构象变化等数据。在此技术基础上发展起来的荧光互相关光谱(fluorescence crosscorrelation spectroscopy,FCCS)突破了FCS技术只能探测单种荧光分子的限制,可以通过分析两种及以上荧光信号来研究分子间的相互作用。由于FCS和FCCS检测的快速精确性,这两种技术被越来越多的应用于生命科学的研究。本文详细阐述了荧光相关光谱和荧光互相关光谱的原理,并对其在生物学尤其是植物细胞生物学中的应用进行了介绍和总结。随着检测技术的改进和数据分析技术的完善,荧光相关光谱和荧光互相关光谱技术将在植物生物学的研究中得到越来越多的应用。
Fluorescence correlation spectroscopy （FCS） is a powerful technique to measure the concentration, diffusion and mobility of molecules on single-molecule level. As a new derivative of FCS, fluorescence cross-correlation spectroscopy （FCCS） can detect crosstalk, and measure the reaction rates and colocalization of two species. FCS and FCCS have been widely applied to life science because of their high accuracy and rapidity. In this paper, we introduce the basic principle of FCS and FCCS and discuss their application to cell biology especially to plant cell biology. Furthermore, we present an overview of technical developments which may benefit from the development of novel labeling and detecting strategies as well as data analyses.
出处
《电子显微学报》
CAS
CSCD
2014年第5期461-468,共8页
Journal of Chinese Electron Microscopy Society
基金
中央高校基本科研业务费专项资金资助(No.BLX 2012039)
长江学者和创新团队发展计划资助项目(No.IRT13047)
