摘要
目的调查温室作业菜农农药暴露情况,筛选农药暴露的早期生物学标志物。方法采用分层整群抽样的方法,于2013年抽取安丘市568名从事温室蔬菜大棚种植者进行问卷调查,依据农药累积暴露强度分为低、中、高3个暴露组;并抽取不从事大棚种植且不接触农药的农民156名作为对照组。检测其外周血全血胆碱酯酶(Ch E)活力、血清超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量。结果中、高暴露组与对照组相比,全血Ch E活力分别下降27.80%和46.23%,血清SOD活力分别下降19.22%和36.63%,MDA含量分别升高152.92%和191.23%,差异均有统计学意义(P<0.05)。菜农全血Ch E活力、血清SOD活力与农药暴露强度呈负相关(r值分别为-0.667,-0.532),MDA含量与农药暴露强度呈正相关(r=0.504),差异均有统计学意义(P<0.05)。结论全血Ch E活力、血清SOD活力和MDA含量能较好地反映农药暴露对温室大棚种植者的早期健康影响,可作为农药早期暴露的生物学标志物。
Objective To understand the pesticide exposure of greenhouse workers, and analyze the biomarkers for greenhouse workers with long-term exposure to pesticides. Methods Cluster random sampling method was used to select 568 greenhouse workers（exposure group） and 156 farmers without exposure to pesticides and greenhouse（control group） in Anqiu, and face to face questionnaire was carried out. According to the level of pesticides exposure, the vegetable greenhouses workers were divided into low, moderate, high exposure groups; At the same time, blood samples were collected to detect the activity of cholinesterase（Ch E） and serum superoxide dismutase（SOD）, and the concentration of malondialdehyde（MDA）. Results Compared with the control group, Ch E activities in moderate and high exposure groups significantly decreased by 27.80% and46.23% respectively. SOD activities in moderate and high exposure groups significantly decreased by19.22%, 36.63%respectively. The MDA concentration significantly increased by 152.92% and 191.23% respectively. Conclusion Ch E activity,serum SOD activity and MDA concentration can be used as the early effect biomarkers of pesticides exposure.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2014年第8期710-712,共3页
Journal of Environment and Health
基金
山东省自然科学基金(ZR2013HQ015)
潍坊医学院科技创新基金(K11QC1004)
