摘要
目的：建立生脉颗粒质量标准控制方法。方法：采用TLC五味子薄层鉴别方法以及HPLC法测定五味子中五味子醇甲的含量。色谱柱：phenomenex Luna C18（150×4.60 mm,5μm）;流动相：甲醇-0.1%磷酸溶液（60∶40）;流速：1.0 m L/min;检测波长：250 nm。结果：薄层鉴别方法简单,阴性无干扰;五味子醇甲在0.0712~2.136μg呈良好的线性关系,r=0.999 9,平均回收率为97.23%;结论：该方法简单、快速、专属性强、可操作性强。
Objective： To establish a method for quality standard of Sheng Mai granules. Methods： The contents of schisandrin in Wu Wei Zi（Schisandra chinensis） were determined by HPLC and distinguished by TLC.Chromatographic column： phenomenex Luna C18（150×4.60 mm, 5 μm）; mobile phase： methyl alcohol-0.1% phosphoric acid solution（60∶40）; flow rate： 1.0 m L/min; detection wavelength： 250 nm. Results： TLC method was simple and the blank test demonstrated no interference. Schisandrin demonstrated better linear relationship in the range between 0.0 712 and 2.136 μg, r=0.9 99 9, average recovery rate was 97.23%. Conclusion： The method is simple, rapid, specific and feasible.
出处
《西部中医药》
2014年第11期38-41,共4页
Western Journal of Traditional Chinese Medicine
