摘要
目的研究高原驻训部队返回人员炎症因子失衡的变化及其意义。方法对21名长期居住平原地区(海拔200 m),平均25岁,初上高原前身体健康,驻训6个月返回后出现高原脱适应症的男性官兵,在上高原前(Control)、返回平原第2天(d2)及第30天(d30)分别抽取空腹静脉血,分离血清,-80℃冻存,ELISA法检测上述返回人员血清中IL-17A及IL-10的含量。结果 d2及d30时间点IL-17A含量及IL-17A/IL-10比率较Control有显著增高(P<0.05),d30时间点IL-17A含量及IL-17A/IL-10比率低于d2时间点(P<0.05);d2及d30时间点IL-10的含量较Control有显著降低(P<0.05),但d30时间点IL-10含量高于d2时间点(P<0.05)。在Control、d2、d30时间点IL-10与IL-17A存在呈正相关(r1=0.948,P<0.05;r2=0.969,P<0.05;r3=0.972,P<0.05);IL-10与IL-17A在d2与Control间、d30与d2间的改变量呈负相关(r4=-0.793,P<0.05;r5=-0.756,P<0.05)。结论高原驻训部队返回人员机体中存在炎症因子失衡的现象,其程度随着返回平原时间的延长而减弱,其炎症因子失衡有所恢复,但其具体机制仍需进一步研究。
Objective To analyze the change in the concentration of IL-17 and IL-10 inflammatory factors among the deadaptation personnel who returned from the plateau.Methods A total 21 healthy males were investigated who averaged 25 years in age, lived permanently in the plains （200 m）, and once stayed to the plateau （Lasha） for 6 months.Their venous blood was collected at three time points：the day before ascending to the plateau（control）, the second day after return to the plains（d2） and the 30th day（d30）, respectively.Their serum was seperated from the whole blood and the level of IL-17A and IL-10 was detected by ELISA method.Results The concentration of IL-17A and the IL-17A/IL-10 ratio were significant increased at d2 and d30, respectively, compared with control （P＆lt;0.05）.Compared with d2, IL-17A and the IL-17A/IL-10 ratio were decreased obviously at d30（P ＆lt;0.05）.The level of IL-10 at d2 and d30 was significantly reduced compared with control （ P ＆lt;0.05）, but increased at d30 compared with d2.Correlative analysis showed that there was a negative correlation in the levels of IL-10 and IL-17A between control, d2 and d30, respectively （r1=0.948, P＆lt;0.05;r2=0.969, P＆lt;0.05;r3=0.972, P＆lt;0.05）.A significant negative correlation was observed in the alteration levels of IL-10 and IL-17A between the three groups（r4=-0.793, P＆lt;0.05; r5=-0.756, P＆lt;0.05）. Conclusion The concentration of inflammatory factors among the plateau deadaptation patients is imbalanced, but it is gradually reduced with time.The mechanism is still not clear.
出处
《军事医学》
CAS
CSCD
北大核心
2014年第10期768-770,779,共4页
Military Medical Sciences
基金
国家科技支撑计划资助项目(2009BAI85B03)
