摘要
目的 肠道病毒71型（EV71）感染引起手足口病（HFMD）临床症状轻重不同的病因尚不清楚，通过比较分析EV71 RNA病毒载量与HFMD临床严重程度的关系，为控制及有效治疗HFMD 提供临床依据。方法 应用实时荧光定量反转录（RT）-PCR技术检测西安市儿童医院2012年1月至2013年9月随机抽取的247例肛拭子EV71病毒核酸阳性的HFMD住院患儿咽拭子标本中EV71 RNA，同时采集患儿的临床资料，包括年龄、性别、临床症状及体征，血常规、空腹血糖、炎性指标、病原学检测，应用 SPSS 13.0软件比较分析其与HFMD 临床严重程度的关系。结果 247例HFMD患儿中，普通病例121例，重症病例126例。2组患儿临床特征比较，肢体抖动、空腹血糖、淋巴细胞及中性粒细胞百分比差异有统计学意义（P均 〈0.05）。247份咽拭子标本EV71的病毒载量大于100 copies/mL，最高者达 1.1×105copies/mL，121例普通病例最高者达9.32×104copies/mL，126例重症病例最高者达 1.1×105copies/mL。2组病毒拷贝数的对数经统计学分析比较，差异无统计学意义（P 〉0.05）。结论 病毒核酸实时荧光定量技术法对咽拭子标本EV71 RNA 定量检测结果直观，方便快捷。EV71型病毒载量高低与HFMD严重程度无明显相关，但需更进一步的大量临床样本证实。
Objective It＇s not clear why the severity of hand-foot-mouth disease （HFMD） caused by entero- virus 71 （EV71） was varied clinically. In this study, the relation between the virus load of EVT1 and the severity of HFMD was analyzed and the foundation for HFMD control and treatment were laid. Methods Two hundred and forty- seven patients with HFMD caused by EV71 from Jan. 2012 to Sep. 2013 in Xi＇an Children＇s Hospital were enrolled and clinical data were collected, including age, gender, clinical symptoms, signs, routine blood count, fasting glucose, C- reactive protein and enterovirus. The EVT1 virus loads of 247 patients with HFMD were measured by real-time PCR as- say. Meanwhile,the effect of EV71 RNA on the severity of HFMD was analyzed. Results In this study, 121 mild cases and 126 severe cases with HFMD limb shaking, fasting glucose, lymphocyte and neutrophil percentage were statistically significant between the 2 groups （ all P 〈 0.05 ）. The counts of EV71 RNA from all of the 247 throat swab samples were more than 100 copies/mL,and the highest virus load was 1.1 × 105 copies/mL. The highest value of EV71 RNA was 9.32 × l04 copies/mL and 1.1 × 105 copies/mL in mild and severe cases, respectively, there was no significant difference between the 2 groups （ P 〉 0.05 ）. Conclusions The results of EVT1 RNA by RT-PCR are visualized and convenient. The virus loads of EV71 are not associated with the severity of HFMD, but further studies need to analyze.
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2014年第22期1700-1702,共3页
Chinese Journal of Applied Clinical Pediatrics
基金
基金项目：陕西省科学技术研究发展项目（2011K12-82）
西安市科学技术局医疗卫生研究项目（SF1208）
