摘要
目的探讨腹泻综合征致泻性大肠埃希菌的血清型分布及耐药性,为临床合理使用抗生素提供依据。方法选取719份未使用抗生素腹泻患者的稀便、水样便、黏脓便或脓血便标本作为研究对象,通过増菌、各种选择性培养基筛选、生化试验,对符合株进行致泻性大肠埃希菌的血清型分型,然后进行药敏试验。结果本次检测共检出65株血清凝集致泻性大肠埃希菌,分属EPEC、EIEC、ETEC,以EPEC为主,占83.08%,共8个血清型,其中血清型O55:H59、O128:H67占58.47%,药物敏感性高的抗生素为CFX(87.7%)、CTX(83.1%)和CIP(86.2%),耐药性高的抗生素为TET(56.9%)、GEN(69.2%)和NAL(52.3%)。结论本地区腹泻综合征中血清学阳性致泻性大肠埃希菌以EPEC为主,常见血清型为O55:H59、O128:H67。腹泻综合征致泻性大肠埃希菌对抗生素的敏感性差异较大,其中检测菌株对CFX、CTX和CIP具有较高敏感性。
Objective To explore the serotype distribution and drug resistance of diarrheagenic Escherichia coli in diarrhea,and provide scientific basis for the reasonable use of antibiotics in clinic. Methods 719 specimens with watery,sticky purulent or bloody purulent stool without the use of antibiotics in diarrhea from May 2012 to April 2013 was selected as research object,which was bacteria-proliferated,filtrated from kinds of selective medium and treated with biochemical test.The fit samples of diarrheagenic Escherichia coli was serotyped and then treated with drug sensitive test.Results 65 strains of serum agglutination diarrheagenic Escherichia coli were detected,which could be assigned to EPEC、EIEC and ETEC respectively.The main part of these strains was EPEC and accounted for 83.08%.There were eight serotypes in total,serotype of O55：H59 and O128：H67 accounted for 58.47%.The antibiotic of high drug sensitivity was CFX（87.7%）,CTX（83.1%） and CIP（86.2%）.The antibiotic of high drug tolerance was TET（56.9%）,GEN（69.2%）and NAL（52.3%）. Conclusion The main serological positive diarrheagenic Escherichia coli of the local diarrhea syndrome is EPEC,the common serotypes are O55：H59 and O128：H67.The distinctions of the sensitivities of diarrheagenic Escherichia coli to antibiotics are large,and CFX, CTX and CIP has a high sensitivity for the detection of strains.
出处
《中国当代医药》
2014年第33期144-146,共3页
China Modern Medicine
关键词
致泻大肠埃希菌
血清型
耐药性
diarrheagenic Escherichia coli
Serum type
Drug resistance
