摘要
为研究中央空调系统真菌繁殖扩散机理及控制空气微生物污染问题,通过温热控制手段,探究不同温热环境下真菌菌落、菌丝生长繁殖规律.以某体育馆中央空调系统过滤器表面真菌为研究对象,通过分离、生理生化实验和分子生物学鉴定确认空调系统过滤器表面的优势菌属种类及数量.对分离鉴定的优势菌属做温热控制实验,分别在25℃恒温及22℃～28℃变温条件下观察菌落和菌丝的生长规律.结果显示,该空调系统过滤器表面真菌优势菌属为青霉属（Penicillium spp.）和枝孢属（Cladosporium spp.）,菌落形成单位分别为600,140cfu/cm^2.温热控制研究表明,无论25℃恒温或22～28℃变温条件,青霉属生长速度都要快于枝孢菌,且菌落直径变化和时间成线性关系;温热控制对青霉属和枝孢菌的生长繁殖具有明显的抑制作用,温热控制技术在抑制空调系统真菌生长上可行.
To explore the propagative and diffused mechanism of fungi in central air conditioning system and control air microorganism contamination problem, growth rhythms of fungal colony and mycelium were researched in different thermal environments by thermal control. Fungi separated from the filter surface of the central air conditioning system in a gym were chosen as the research objects. By isolation, physiology and biochemistry experiments, and molecular biological identification, dominant fungi on the filter of central air conditioning system were confirmed. In order to conduct thermal control experiment of separated dominant fungi, growth rhythms of fungal colony and mycelium were observed at temperature of 25℃ and 22～28℃, respectively. As a result, it was shown that the dominant fungi were Penicillium spp. and Cladosporium spp., colonies were 600 and 140 cfu/cm^2 respectively. It was indicated that regardless of constant or variable temperature conditions, the reproduction rate of Penicillium spp. was faster than Cladosporium spp., with a liner correlation between colony diameter and time. Penicillium spp. and Cladosporium spp were obviously restrained by temperature change control. Thermal control was feasible for restraining fungal growth in air conditioning system.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2014年第11期2917-2921,共5页
China Environmental Science
基金
国家自然科学基金资助项目(51308088)
高等学校博士点专项基金(20120041120003)
北京市重点实验室研究基金(NR2013K05)
大连理工大学基本科研业务费专项(DUT14QY24)
关键词
中央空调系统
过滤器
真菌
分离鉴定
温热控制
central air conditioning system
filter
fungi
isolation and identification
thermal control
