摘要
以溶解性为指标,采用单酶(Alcalase 2.4 L)和双酶(Alcalase 2.4 L+Flavourzyme 500 MG)2种方法水解谷朊粉,在单因素试验的基础上通过正交试验,优化了单酶和双酶水解的最佳条件,并分析了最优酶解条件下得到的2种产物的抗氧化活性、相对分子质量分布及其氨基酸组成。结果表明,单酶和双酶水解物的最大溶解性分别达到了61.38%和82.21%。水解物质量浓度为3.0 mg/m L时,单酶水解物的还原力、DPPH自由基、超氧阴离子、羟自由基的清除率分别达到0.81、71.82%、56.83%和70.82%,而双酶水解物相应的指标分别达到1.01、85.33%、74.84%和84.33%。结果表明,双酶水解不仅能提高谷朊粉的溶解性,而且也能明显提高其抗氧化活性。双酶水解物中小分子肽(分子质量<1 500 u)和疏水性氨基酸的质量分数分别达到61.63%和33.74%,均高于单酶水解物,表明谷朊粉酶解物的抗氧化活性与小分子肽含量、疏水性氨基酸呈现正相关。
Taken solubility as a response value, wheat gluten has been hydrolyzed with protease through two treatments [ single enzyme （ Alcalase ） and double （ Alcalase - flavor） enzyme ]. The optimal enzymatic hydrolysis conditions of single enzyme and double enzyme were established by single factor and orthogonal test. Under the opti- mal enzymatic conditions, the antioxidant activity, molecular weight and amino acid compositions of the single and the double enzymatic hydrolysates have been measured. The resuhs showed that the solubility of single and double enzy- matic hydrolysate was 61.38% and 82.21% respectively. The reducing power, DPPH ~ , superoxide anion and hy- droxyl radical scavenging activity of single enzymatic hydrolysates with mass concentration of 3.0 mg/mL reached 0.81, 71.82% , 56.83% and 70.82% respectively. The corresponding reached 1.01, 85.33%, 74.84% and 84.33% respectively. The results sis could not only improve solubility of wheat gluten hydrolysates, but also indictors of double enzymatic hydrolysate showed that the double enzymatic hydroly- significantly develop the antioxidant activi-ty. The mass ratio of small molecular weight peptides （ MW 〈 1 500 u） and hydrophobic amino acids in double enzy- matic hydrolysate were 61.63% and 33.74% respectively, which were higher than those of single enzymatic hydroly- sate. It indicated that there was a positive correlation between antioxidant activity of wheat gluten hydrolysates and the content of small molecular weight peptides or hydrophobic amino acids.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2014年第11期7-13,共7页
Journal of the Chinese Cereals and Oils Association
基金
国家自然科学基金(31071517)
关键词
谷朊粉
酶解
抗氧化活性
分子质量
氨基酸
wheat gluten, enzymatic hydrolysis, antioxidant activities, molecular weight, amino acids
