摘要
目的 利用同步辐射吸收成像技术（SRμCT）观察大鼠脊髓急性损伤后微血管的三维形态学改变.方法 将8只SD大鼠随机均分为损伤组和对照组,损伤组以改良Allen＇s打击法建立急性脊髓损伤模型.次日,所有大鼠行升主动脉血管造影术后低温保存脊髓标本.脊髓标本于上海光源利用SRμCT进行图像扫描.结果 SRμCT清晰直观地呈现了脊髓完整的血供网络结构.与对照组对比,损伤组脊髓微血管有明显的缺失、断裂和扭曲,血管数目和直径均有显著下降.损伤组平均血管数目为（77.25±16.80）个,对照组为（168.30±12.79）个;损伤组平均血管直径为（16.270±1.201）μm,对照组为（20.210±1.094） μm.各指标差异均有统计学意义（P＜0.05）.结论 SRμCT可对小动物脊髓微血管三维结构进行高分辨率成像.脊髓内微循环网络结构在急性脊髓损伤后显著破坏,严重影响脊髓局部的供血循环.
Objective Using the synchrotron radiation-based micro-computed tomography （SRμCT） to observe the changes of three-dimensional micro-vascular morphology structure after acute spinal cord injury in rat.Methods Eight male Sprague-Dawley rats were randomly divided into control group and injury group （n =4）.Injury group were deal with improved Allen＇ s weight dropping to establish the acute spinal cord injury model.All rats were deal with ascending aorta angiography in the next day.Freezing the specimens for 24 hours and then scanned them in Shanghai Institute of Applied Physics,Chinese Academy of Sciences.Processed and reconstructed the Data to three-dimensional images.Results The SRμCT can show the micro-vascular structure of spinal cord in rat completely and clearly.Combining with control group,we can see the lack and fracture of the micro-vessels of spinal cord in injury group significantly.The number and diameter of the micro-vessels were decreased obviously.The average number of vascular in injury group was 77.25 ± 16.80,and the control group was 168.30 ± 12.79 ; The average diameter of vascular in injury group was （16.270 ± 1.201） μm,but the control group was （20.210 ± 1.094） μm.Significant differences were existed between control group and injury group （P 〈 0.05）.Conclusion The SRμCT can get the three-dimensional and high resolution imaging of micro-vessels in small animal＇ s spinal cord.The micro-circulation network structure of spinal cord was prominently destroyed after acute spinal cord injury.The blood circulation of spinal cord in injury areas and its vicinity was serious damaged.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第11期2534-2536,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（81171698、81371956、81301542）
关键词
同步辐射
吸收成像
脊髓损伤
微血管
形态学
Synchrotron radiation
Spinal cord injury
Micro-vessels
Morphology
