摘要
[目的]了解安徽省中等规模种猪场猪伪狂犬病病毒（PRV）的感染情况。[方法]2013年8月至11月，开展了中等规模种猪场猪伪狂犬病群流行率和风险因素的横断面研究。通过简单随机抽样方法，选取了380个中等规模种猪场；用发现疫病的抽样策略，在每个种猪场采集了5份母猪血清，使用IDEXX公司猪伪狂犬病病毒gE-ELISA试剂盒进行了抗体检测。同时开展了问卷调查，并对种猪场之间PR传播潜在的29个风险因素进行了统计学分析。[结果]2013年安徽省中等规模种猪场猪伪狂犬病的群流行率为34.1%（95%置信区间29.3%~38.9%）；没有灭鼠措施（OR=2.5，95%置信区间：1.4~3.7，P＆lt;0.001）、购入种猪未经检测（OR=2.2，95%置信区间：1.1~4.2，P=0.024）、未免疫含PR 6种以上猪病（OR=2.0，95%置信区间：1.2~3.1， P=0.004）是2013年安徽省中小规模种猪场感染PRV的风险因素。Logistic 回归拟合度较好（P＆gt;0.05），ROC曲线下面积为0.68（95%置信区间：0.62-0.73）。[结论]安徽省中等规模种猪场场间PR流行率较高，建议购入种猪前需进行检测，免疫含PR等6种以上猪病疫苗并定期灭鼠。
To get a better understanding of the infection status of pseudorabies virus（PRV） in middle-scale pig farms in Anhui province,a cross-sectional study was conducted from August to November 2013 to estimate the prevalence and identify risk factors of PRV infection in middle-scale pig farms in Anhui province. 380 farms were selected randomly in the study. Sample size was determined using a risk-based sampling strategy for the detection of virus, and all the samples were tested using gE-ELISA kits for antibodies to PRV gE protein. Questionnaires were fi lled out during the process of sampling for the purpose of risk factor identifi cation using multiple regression analysis. The results showed that farm prevalence of PRV was 34.1%（95%CI：29.3%~38.9%）in middle-scale pig farms in Anhui province. Three risk factors（no mouse-proof measure（OR=2.5,95%CI： 1.4~3.7,P〈0.001）,no PRV test in introduction of pigs（OR=2.2,95%CI： 1.1~4.2,P=0.024） and no vaccinations against the six major pig diseases in farm（OR=2.0, 95%CI： 1.2~3.1,P=0.004））,were identified by multiple regression analysis. The ROC curve（0.68,95% CI： 0.62~0.73） indicated that the study had a relative high capcity for predicting the true positive value. It was conclued that the seroprevalence of PRV in middle-scale pig farms was high and more specifi c control measures should be taken in the future,such as enhancing quarantine for PRV during the introduction of breeding pigs,optimizing the vaccinaiton protocols against the six major pig diseases and installing mouse-proof facilities on the farm.
出处
《中国动物检疫》
CAS
2014年第11期16-20,47,共6页
China Animal Health Inspection
