摘要
目的探讨微柱凝集抗人球蛋白试验(AGT)在贫血患者输血前的应用价值。方法对234例贫血患者输血前进行微柱凝集直接抗人球蛋白试验(DAT),阳性者再利用标准抗体筛查细胞进行间接抗人球蛋白试验(IAT)。结果 234例标本中DAT阳性者10例,经抗体筛查,其中IAT阳性者3例。结论微柱凝集AGT是一种灵敏度较高的可靠方法,该方法可以检测贫血是否由抗体致敏红细胞所导致,对指导临床输血具有决定意义。
Objective To study the application of antiglobulin test(AGT)with microcolumn gel assay before the blood transfusion of anemia.Methods Direct antiglobulin test(DAT)with microcolumn gel assay was proceeded in 234 patients with anemia before blood transfusion.Indirect antiglobulin test(IAT)proceeded by using the standard cells for antibody screening in the patients having positive DAT results.Results There were 10 cases of DAT positive samples among all of the 234 samples,and 3of them were IAT positive during antibody screening.Conclusion AGT with microcolumn gel assay is a highly sensitivity and reliable test.It can suggest whether the anemia is caused by antibody-sensitized erythrocytes,and it has a decisive significance in guiding the clinical blood transfusion.
出处
《国际检验医学杂志》
CAS
2014年第21期2937-2938,共2页
International Journal of Laboratory Medicine
关键词
抗人球蛋白试验
微柱凝集法
贫血
输血
antiglobulin test
microcolumn gel assay
anemia
blood transfusion