摘要
目的探讨白细胞介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)对髓核细胞的基质金属蛋白酶-28(matrix metalloproteinase-28,MMP-28)是否具有调控作用。方法培养人体正常髓核细胞,将3个不同浓度的IL-1β(0 ng/mL、10 ng/mL、50 ng/mL)和3个不同浓度的TNF-α(0 ng/mL、50 ng/mL、100 ng/mL)分别与体外扩增的第3代髓核细胞联合培养72 h,采用实时聚合酶链反应(real time-polymerase chain reaction,RT-PCR)法测定MMP-28 mRNA的转录水平,采用单因素方差分析法比较转录水平的差异。结果不同浓度(0 ng/mL、10 ng/mL、50 ng/mL)的IL-1β与髓核细胞共培养72 h后,各组间MMP-28mRNA转录水平(0.325±0.019、0.343±0.018、0.343±0.018)差异无统计学意义(P>0.05)。不同浓度(0 ng/mL、50 ng/mL、100 ng/mL)TNF-α与髓核细胞共培养72 h后,各组间MMP-28 mRNA转录水平(0.325±0.019、0.515±0.02、0.610±0.012)差异有统计学意义(P<0.01),且高浓度组MMP-28 mRNA转录水平较低浓度组明显升高(P<0.01)。结论 IL-1β对正常人体髓核细胞MMP-28转录无调控作用,而TNF-α可以上调MMP-28mRNA转录水平,且呈浓度依赖性。
Objective To investigate whether matrix metauoproteirase-28 （ MMP-28 ） can be regulated by interleubin-β（ IL-1β） and tumor necrcsis factor-α（ TNF-α） in human nucleus pulposus cells after coculture .Methods After the third gen-eration of human nucleus pulposus cells were stimulated with different concentration of IL -1β（0 ng/mL,10 ng/mL,50 ng/mL） and TNF-α（0 ng/mL,50 ng/mL,100 ng/mL） 72 h in vitro, MMP-28 mRNA was quantified by RT-PCR,the difference between each concentration were compared by one-way analysis of variance .Results There was no sign indicated that the various concentration of IL-1β（0 ng/mL,10 ng/mL,50 ng/mL） can affect human nucleus pulposus cells MMP-28 transcrip-tion （0.325 ±0.019 vs.0.343 ±0.018 vs.0.343 ±0.018, P〉0.05）.But cytokines TNF-αcould induce the expression of MMP-28, various concentration groups present significant difference （0.325 ±0.019 vs.0.515 ±0.02 vs.0.610 ±0.012, P〈0.01）, and with the concentration increasing , the effect was more prominent （0.610 ±0.012 vs.0.515 ±0.02, P〈0.01）.Conclusion IL-1βdoesn’t affect human nucleus pulposus cells MMP-28 transcription in vitro.However, TNF-αcan up regulate the MMP-28 mRNA of the nucleus pulposus cells and the effects are strengthened by enhancing concentration .
出处
《脊柱外科杂志》
2014年第4期248-251,共4页
Journal of Spinal Surgery
基金
江苏省"六大人才高峰"高层次人才基金(2012-wsn-081)
