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不同基因型花生EMS诱变条件分析及突变系筛选 被引量:12

Analyzing EMS Mutagenesis Conditions of Different Genotypes Peanut and Screening of Mutant Lines
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摘要 为明确不同基因型花生最佳甲基硫酸乙酯(EMS)诱变条件,以"四粒红"和"白沙1016"花生品种为材料,在0.30%、0.90%EMS溶液中浸泡3、5、7和9 h,进行发芽试验。结果表明:以致死量达到50%为标准,多粒型花生的最佳处理条件是0.90%EMS溶液诱变7h,珍珠豆型花生则为0.90%EMS溶液诱变9 h,多粒型花生对EMS响应明显。在M3、M4代突变系中,多粒型筛选出YD35、YD3、和YD223个高产株系,最高增产565 kg/hm2,珍珠豆型筛选出YZ4、YZ2和YZ13个高产株系,最高增产570 kg/hm2。 In order to clarify the best EMS mutagenesis conditions of different peanut genotypes,with ‘ Silihong’ and‘ Baisha1016’ as materials,the germination experiments were conducted at 0.30%,0.90% EMS solution soaking for 3,5,7 and 9 h.Lethal to 50% of the standard,the results showed that the optimum treatment conditions of multi-grain type peanut is soaking into 0.90% EMS solution for 7 hours,and the vulgaris type peanut need soaking into 0.90% EMS solution for 9 hours.Multi-grain type peanut was significantly response for EMS.In the M3 and M4 generations mutant lines,multi-grain type peanut had selected three high-yield strains,YD35,YD3 and YD22,and the highest incremental was 565 kg/hm2 in yield.Vulgaris type peanut also had screened three high-yield strains,YZ4,YZ2 and YZ1,and the highest incremental was 570 kg/hm2 in yield.
出处 《安徽农业科学》 CAS 2014年第33期11778-11781,共4页 Journal of Anhui Agricultural Sciences
基金 国家花生产业技术体系项目(CARS-14) 吉林省科技发展计划项目(20120210) 吉林省自然科学基金项目(201215195)
关键词 花生 多粒型 珍珠豆型 EMS诱变 突变系 Peanut Multi-grain type Vulgaris type EMS mutagenesis Mutant lines
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