摘要
噬菌体抗体库技术是将目的基因克隆到噬菌体的外壳蛋白基因组中,转染宿主菌后,使目的基因编码蛋白和外壳蛋白以融合蛋白的形式表达,呈现在噬菌体表面,通过一系列的筛选获得特异性噬菌体抗体分子。介绍了噬菌体抗体库技术在水产养殖过程中的疾病防控、水环境监测、水产品安全控制以及与抗体芯片结合研究等方面的研究进展。
Target gene are displayed on the surface of filamentous bacteriophage by genetic fusion to a coat protein of phage is the basic principle of phage antibody library technology.The most characteristic of it is a direct physical link from genotype to phenotype and the specific antibody of the Phage could be obtained by screening of enrichment.In this paper,recent research progress on disease control,water environment monitoring,aquatic products safety control,and combination with the antibody microarray in the aquaculture industrv has beenreviewed.
出处
《安徽农业科学》
CAS
2014年第33期11740-11742,共3页
Journal of Anhui Agricultural Sciences
基金
福建省海洋与渔业厅项目(201212140006)
福建省教育厅项目(JA11150)
集美大学创新团队基金项目(2010A001)
关键词
噬菌体抗体库
水产养殖
研究进展
Phage antibody library
Aquaculture
Research progress
