摘要
细菌菌影(bacterial ghost,BG)是革兰阴性生菌在噬菌体PhiX174的裂解基因E的作用下形成不含核酸、核糖体等胞质内容物的细菌空壳。这种细菌空壳保留了与天然细菌一样的完整外膜结构,且不具有活菌样的致病作用,可作为疫苗无需佐剂就能诱导机体产生体液免疫应答和细胞免疫应答。菌影内部及外膜上可装载DNA、抗原和药物等异源物质,易被机体免疫细胞识别捕获,使其成为一种新型的生物递送载体。另外,菌影具有制备简单,易于保存等优点。
Bacterial ghost （BG） is an empty bacterial envelope of gram-negative bacteria devoid of cytoplasmic content, and produced by controlled expression of bacteriophage PhiX174 lysis gene E. BG keeps intact bacterial outer membrane structure, such as flagella, LPS and outer membrane proteins, making bacterial easy to adherence to the human or animal cell receptors, so it can be used as vaccines without adjuvant to induce strong humoral immune response and cellular immune response. membrane, the periplasmic space and the inner membrane to load DNA, antigen and drugs delivery vector as it exhibits potency with BG has many spaces, such as the outer other substance. BG also can be used as regard to target antigens and large capacity compared to conventional approaches. In addition, BG can be provided with a foreign antigen, nucleic acid or other ingredients to construct combined vaccine for human or animal health services. BG has wide application prospect in the prevention and treatment of disease.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2014年第9期108-112,共5页
China Biotechnology
基金
国家自然科学基金资助项目(31100652)
关键词
细菌菌影
疫苗
递送载体
Bacterial ghost Vaccine Delivery vector
