摘要
目的：建立同时测定鹿蹄草中齐墩果酸和熊果酸含量的方法。方法：采用反相高效液相色谱法。色谱柱为Kromasil C18（250 mm×4.6 mm,5μm）,流动相为甲醇-水-磷酸（88∶12∶0.15,V/V/V）,流速为0.9 ml/min,柱温为30℃,检测波长为210 nm。结果：齐墩果酸进样量在0.462-4.620μg时,与峰面积积分值呈良好的线性关系（r=0.99998,n=6）,平均加样回收率为98.6%,RSD=2.1%（n=6）;熊果酸进样量在1.064-10.640μg时,与峰面积积分值呈良好线性关系（r=0.99999,n=6）,平均加样回收率为98.5%,RSD=2.2%（n=6）。结论：该方法操作简单、结果准确、重复性好,适用于鹿蹄草中齐墩果酸和熊果酸含量的同时测定。
OBJECTIVE： To establish a method for the content determination of oleanolic acid and ursolic acid in Pyrolae hor- ha. METHODS： RP-HPLC method was adopted. The separation was achieved on a Kromasil C18（250 mm×4.6 mm, 5 μm） column with mobile phase consisted for methanol-water-phosphoric acid （88 ： 12 ： 0.15, V/V/V） at the flow rate of 0.9 ml/min. The column temperature was set at 30℃, and the detection wavelength was maintained at 210 nm. RESULTS： The linear range of oleanolic ac- id were 0.462-4.620 pg （r=0.999 98, n=6） with an average recovery of 98.6% （RSD=2.1% ,n：6）; the linear range of ursolic acid were 1.064-10.640 pg（r=0.999 99,n=6） with an average recovery of 98.5% （RSD：2.2% ,n=6）. CONCLUSIONS： The method is simple, accurate and repeatable, and is suitable for content determination of oleanolic acid and ursolic acid in P. callian- thr7
出处
《中国药房》
CAS
CSCD
2014年第43期4074-4076,共3页
China Pharmacy
基金
国家高技术研究发展计划(863计划)课题项目(No.2002AA2Z3217)
