摘要
目的：研究齐墩果酸对氧化损伤模型人脐静脉内皮细胞（HUVECs）的保护作用。方法：分别以含10%胎牛血清DMEM高糖培养液[含氧化低密度脂蛋白（ox-LDL,质量浓度分别为0、25、50、100、200、400μg/ml）]培养HUVECs,CCK-8法检测细胞活性以筛选复制模型最适质量浓度。以0、10、20、40、60、80、100μmol/L齐墩果酸培养HUVECs,CCK-8法检测细胞活性以考察齐墩果酸试验最适浓度。以5、10、20、40μmol/L齐墩果酸作用于氧化损伤模型HUVECs（100μg/ml ox-LDL诱导）,CCK-8法检测细胞活性,测定一氧化氮（NO）、一氧化氮合酶（NOS）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-PX）水平。结果：复制模型ox-LDL最适质量浓度为100μg/ml;齐墩果酸试验最适浓度范围为0~40μmol/L;5、10、20、40μmol/L齐墩果酸可增加氧化损伤模型HUVECs存活率,增强CAT、GSH-PX、NOS活性,增加NO含量。结论：齐墩果酸能够保护ox-LDL氧化损伤的HUVECs,其保护机制与增强CAT、GSH-PX、NOS抗氧化酶活性有关。
OBJECTIVE：To study the protective effects of oleanolic acid（OA）oxidative damaged human umbilical vein endothe- lial cells （HUVECs）. METHODS： HUVECs were cultured with DMEM high glucose medium of 10% serum [containing ox-LDL 0, 25, 50,100, 200 and 400μg/ml] ; the activity of cells was detected by CCK-8 assay to screen optimal concentration of medium. HUVECs were cultured with OA （0, 10, 20, 40, 60, 80 and 100μmol/L）, and the activity of cells was detected by CCK-8 assay to screen optimal concentration of OA. The oxidative damaged HUVECs （induced by 100 μg/ml ox-LDL） were treated with OA （5, 10,20,40 μmol/L）,and the activity of cells was detected by CCK-8 assay; the levels of NO, NOS, CAT and GSH-PX were detected. RESULTS： For inducing model, the optimal concentration of ox-LDL was 100 μg/ml, and that of OA was 0-40μmol/L; 5, 10, 20, 40μmol/L OA enhanced the survival rate of HUVECs, enhanced the activities of CAT, GSH-PX and NOS and in- creased NO levels. CONCLUSIONS： OA can protect the ox-LDL oxidative damage HUVECs, and the protective mechanism may be associated with the improvement of CAT, GSH-PX, NOS activity.
出处
《中国药房》
CAS
CSCD
2014年第43期4033-4035,共3页
China Pharmacy
基金
国家自然科学基金资助项目(No.81274126)
关键词
齐墩果酸
人脐静脉内皮细胞
动脉粥样硬化
氧化损伤
Oleanolic acid
Human umbilical vein endothelial cells
Atherosclerosis
Oxidative damage
