摘要
目的 评价腹腔注射水溶性脂聚体（WSLP）/含2B亚基的N-甲基-D-天冬氨酸受体（NR2B） siRNA复合物对大鼠神经病理性痛的影响.方法 健康雄性SD大鼠84只,6周龄,体重180～ 200 g,采用随机数字表法分为7组（n=12）：空白对照组（C组）、假手术组（S组）、神经病理性痛组（NP组）、WSLP/NR2B siRNA组（siWSLP组）、WSLP/阴性对照siRNA组（ncWSLP组）、PE[/NR2B siRNA组（PEI组）和WSLP组.C组不给予任何处理,S组仅暴露L5脊神经但不结扎,NP组、siWSLP组、ncWSLP组、PEI组及WSLP组采用结扎L5脊神经的方法制备大鼠神经病理性痛模型,于模型制备后第10天分别单次腹腔注射WSLP/NR2B siRNA、WSLP/阴性对照siRNA、PEI/NR2B siRNA及WSLP复合物2ml.于术前1d、术后7d及腹腔给药后3、7、14及21 d时随机取6只大鼠测定机械缩足反应阈（MWT）和热缩足反应潜伏期（TWL）;于腹腔注射后3d余6只大鼠处死后取脊髓,采用PCR法检测脊髓NR2B mRNA表达水平,采用Western blot法检测脊髓NR2B蛋白的表达水平.结果 与C组比较,其余组术后7d及腹腔给药后3、7和21 d时MWT降低,TWL缩短,腹腔给药后3d时脊髓NR2B mRNA及其蛋白的表达上调（P＜0.01）;与NP组比较,siWSLP组腹腔给药后3和7d时MWT升高,TWL延长,腹腔给药后3d时脊髓NR2B mRNA及其蛋白的表达下调（P＜0.01）,PEI组、ncWSLP组、WSLP组及S组各指标差异无统计学意义（P＞0.05）.结论 腹腔注射WSLP/NR2B siRNA复合物可有效减轻大鼠神经病理性痛.
Objective To evaluate the effect of intraperitoneal water soluble lipopolymer （WSLP）/ N-methyl-D-aspartate receptor subunit 2B （NR2B） siRNA compound on the neuropathic pain （NP） in rats.Methods Eighty-four healthy male Sprague-Dawley rats,aged 6 weeks,weighing 180-220 g,were randomly divided into 7 groups （n =12 each） using a random number table：control group （group C）,sham operation group （group S）,NP group,WSLP/NR2B siRNA group （siWSLP group）,WSLP/negative control siRNA group （ncWSLP group）,PEI/NR2B siRNA group （PEI group） and WSLP group （WSLP group）.NP was produced by ligation of the left L5 spinal nerve.In group S,the left L5 spinal nerve was only exposed,but not ligated.In group C,the rats underwent no treatment.Groups siWSLP,ncWSLP,PEI and WSLP received single intraperitoneal injection of WSLP/NR2B siRNA,WSLP/negative control siRNA,PEI/NR2B siRNA and WSLP compound 2 ml,respectively,at 10 days after NP.At 1 day before operation,7 days after operation,and 3,7,14 and 21 days after intraperitoneal injection,6 rats in each group were chosen randomly to measure mechanical paw withdrawal threshold （MWT） and thermal paw withdrawal latency （TWL）.At 3 days after intraperitoneal injection,the left 6 rats in each group were sacrificed and the spinal cord was removed for detection of NP2B mRNA expression （using PCR） and NR2B expression （by Western blot）.Results Compared with group C,MWT was significantly decreased,and TWL was shortened on 7 days after operation and 3,7 and 21 days after intraperitoneal injection,and the expression of NR2B mRNA and protein was down-regulated on 3 days after administration in the other groups.Compared with group NP,MWT was significantly increased,and TWL was prolonged on day 3 and 7 after intraperitoneal injection,and the expression of NR2B mRNA and protein was down-regulated on day 3 after administration in siWSLP group.Conclusion Intraperitoneal WSLP/NR2B siRNA compound can effectively relieve the NP in rats.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2014年第9期1082-1085,共4页
Chinese Journal of Anesthesiology
基金
国家自然科学基金（81371233）
广东省自然科学基金（S2012010009271）
