摘要
目的:研究大孔吸附树脂分离纯化虎杖中白藜芦醇的工艺条件及参数。方法:利用白藜芦醇显酸性的特点,采用碱液洗脱树脂法分离纯化白藜芦醇。考察6种树脂、碱的浓度以及洗脱流速等因素对白藜芦醇分离纯化的影响。结果:选择NKA-Ⅱ树脂,NaOH洗脱液浓度0.5%,流速为1 BV/h,经过去盐后,白藜芦醇纯度达到70%以上。结论:该工艺适用于对白藜芦醇有吸附的树脂,经过一次上柱就可以与原料中其他干扰成分分离,适合大规模生产。
Objective：To explore purification techniques of Resveratrol by macroporous adsorption resin. Methods：As resveratrol is acidic, lye elution resin separation and purification was used. The impact of resin types, NaOH concentration, flow rate on purification results were explored. Results：The optimized purification techniques was： NKA-Ⅱ resin, eluent concentration of NaOH 0.5%, flow rate = 1BV/h, after desahing, resveratrol purity 〉 =70%. Conclusion：The optimized purification techniques is applicable to adsorption resin that is resveratrol adsopting, separation with other ingredients can be achieved by one flow, it is suitable for mass production.
出处
《世界中医药》
CAS
2014年第10期1361-1363,1366,共4页
World Chinese Medicine
关键词
白藜芦醇
虎杖
大孔吸附树脂
碱
Resveratrol
Cuspidatum
Macroporous resin
Alkali
