摘要
目的 采用“跑台＋撞击”交替刺激踝关节的方式制作大鼠踝前撞击综合征模型,并进行相应的评价.方法 将40只SD大鼠随机分为空白组、跑台组、撞击组和跑台＋撞击组4组,每组各10只,空白组不处理,跑台组大鼠置于跑台,每日30 min,每周5d,跑台平板斜度30°,履带传输速度12 m/min,进行4周跑台训练;撞击组大鼠固定右侧踝关节后,采用撞击装置撞击踝关节,单次撞击能量4.5 J,每日撞击300次,每周5d,连续30 d.跑台＋撞击组大鼠采用跑台与撞击仪共同处置,仪器参数设置同上两组.实验的10d、20 d、30d对各组大鼠进行踝关节屈伸疼痛评分、踝关节体积测定,实验30 d进行微计算机断层扫描技术（Micro CT）、病理学检测.结果 跑台＋撞击组踝关节屈伸疼痛评分、踝关节体积在各个检测节点均显著高于其他两组,差异具有统计学意义（P＜0.05）;跑台＋撞击组踝关节Micro CT及软骨标本大体观察以轻度或中度病变为主,严重程度及评分与其余各组有显著性差异（P＜0.05）.结论 跑台＋撞击可以制作较为理想的踝前撞击综合征模型,其病情分期类似于骨性关节炎的早期及中期.
Objective To reproduce a rat model of anterior ankle impingement syndrome by treadmill exercise or/and impacted device, and to evaluate the model by different methods. Method Fourty SD rats were randomly divided into 4 groups： blank control group, exercise group, impact group, exercise ＆ impact group. The rats in the exercise group were received treadmill exercise 5 days per week for 4 weeks according to the following schedule： 12 m/min for 30 minutes every day, slope grade of the treadmill was adjusted at 30°; impact group were impacted by self-made special device which gave energy of 4.5 J in the impacted area, 300 times per day; exercise ＆ impact group were received both treadmill exercise and impact, and the parameters were the same as above. During the experiment of the 10,20 and 30 days,the flexion and extension of ankle joint pain score, ankle joint volume were detected, the Micro CT and pathological observation were applied to evaluate the model at 30 days. Result The flexion and extension of ankle joint pain score, ankle joint volume in exercise ＆ impact group were significant difference compared with the single induced groups, most data of the Micro CT test and the auricular cartilage pathological stage in exercise ＆ impact group were mild or moderate injury, which was significant difference compared with the single induced groups. Conclusion Treadmill exercise combined with impacted device can reproduce an ideal rat model of anterior ankle impingement syndrome, which similar to early stage or middle stage of osteoarthritis.
出处
《实验动物与比较医学》
CAS
2014年第5期360-364,共5页
Laboratory Animal and Comparative Medicine
基金
上海市科学技术委员会科研计划项目（编号：11140901800）
关键词
踝前撞击综合征
大鼠
模型
评价
Anterior ankle impingement syndrome
Rats
Model
Evaluation
