摘要
目的检测CD74蛋白在结肠腺癌中的表达,探讨其表达的临床意义及与预后的关系。方法应用免疫组化PV 9000法对53例结肠腺癌进行CD74免疫染色,同时选取结肠腺瘤38例、结肠炎11例及正常结肠黏膜26例作为对照,分析结肠腺癌中CD74的表达情况及与临床病理参数的关系。结果结肠腺癌CD74的阳性率(79%)高于各对照组,与正常黏膜组(12%)和结肠炎组(31%)比较差异显著,与结肠腺瘤组(68%)比较差异不显著。CD74在结肠腺癌中的阳性强度高于正常组、结肠炎组和结肠腺瘤组,且差异显著。结肠腺癌中CD74阳性检出率与肿瘤组织学分级和分期有关,与患者年龄、性别、肿瘤大小、肿瘤部位、浸润深度、有无淋/巴结转移无关;CD74阳性强度仅与肿瘤分化程度有关。CD74阳性的结肠腺癌患者5年生存率(57.1%)明显低于CD74阴性患者(81.8%),其中CD74阳性强度高的患者生存期比CD74阳性强度低的患者生存期短,Kaplan-Meier生存曲线分析差异显著。COX回归表明,CD74在结肠腺癌中的表达为影响结肠腺癌预后的独立危险因素(P<0.01)。结论CD74表达增高可能在结肠腺癌发生、发展中起重要作用,CD74阳性的结肠腺癌预后差。
Purpose To investigate the expression and clinical significance of CD74 in colon adenocarcinoma,and discuss its impact on the prognosis.Methods 53 cases of paraffin-embedded colon adenocarcinoma specimens were evaluated by PV 9000 immunohistochemical procedures for expression of CD74.Meanwhile,38 cases of colonic adenoma,11 cases of colitis and 26 cases of normal colon were randomly selected as a control.The relation of CD74 immunohistochemical staining results and clinicopathological parameters were analyzed.Resuts The positive expression rate of CD74 in colonic carcinoma group （79％） was higher than the control group.The difference were statistically significant among colonic carcinoma,colitis （31％） and normal colon （12％）.There were no statistically significant differences between colonic carcinoma and colonic adenoma in positive rate of CD74.The intensity of stain in colon carcinoma was significantly stronger than normal colon,colitis and colonic adenoma.CD74 in high positive expression of colon carcinoma was associated with the grade of tumor differentiation and stage of disease,but not with patients’ age,gender,tumor size,tumor location,infiltration depth,lymph node metastasis.The CD74 stain intensity was only related to the grade of tumor differentiation.Kaplan-Meier survival curve showed that the five-year survival rate of colon cancer patients with CD74 positivity（57.1％） was significantly lower than CD74-negative patients （81.8％）,and the difference had statistical significance.And the survival time in CD74 positived colon carcinoma patients with high stain intensity was shorter than the patients with low CD74 stain intensity,there also had statistical significance（P =0.012）.COX regression showed that CD74 expression in colonic carcinoma was the independent risk factor for the development of colonic cancer prognosis.Conclusions Increased expression of CD74 may play an important role in colon carcinoma development.Colon cancer patients with CD74 positive-expression have a
出处
《诊断病理学杂志》
CSCD
北大核心
2014年第10期614-617,621,共5页
Chinese Journal of Diagnostic Pathology
基金
四川省卫生厅科研课题基金项目(120025)
关键词
结肠癌
CD74
免疫组化
预后
Colon carcinoma
CD74
Immunohistochemistry
Prognosis
