摘要
目的:探讨Lin28A/B亚型对肝癌细胞凋亡的影响,揭示其在肝癌发生发展过程中的作用.方法:Real-time PCR和Western blot分别检测Lin28A/B亚型在肝癌细胞系中的表达变化;Real-time PCR和Western blot检测siRNA分别干扰Lin28A/B亚型后二者在肝细胞癌中的表达;MTT法分别Lin28A/B siRNA转染肝癌细胞48、72 h后对肝癌细胞增殖的影响,FACS检测二者对肝癌细胞凋亡的作用.结果:Lin28A和Lin28B在肝癌细胞中的表达明显上调(P<0.05;P<0.01),其中Lin28B在肝癌细胞中表达增加更加明显;siRNA可降低Lin28A/B在肝癌细胞中的表达(P<0.05);Lin28A/B siRNA均可抑制肝癌细胞的增殖,诱导肝癌细胞凋亡率显著增加,且随着转染时间的延长而进一步增加(P<0.05;P<0.01);其中Lin28B siRNA对肝癌细胞的作用优于Lin28AsiRNA(P<0.05).结论:Lin28A/B两种亚型均参与肝癌细胞的发生、发展,其中Lin28B亚型在诱导肝癌细胞凋亡中起主要作用,而Lin28A可能起到协同作用.
AIM： To explore the role of Lin28A/B subtypes in apoptosis of hepatocellular carcinoma （HCC） cells, and to analyze the mechanism of action of Lin28A/B in tumorigenesis of HCC. METHODS： The mRNA and protein expres- sion of Lin28A/B in HCC cells was detected by real-time PCR and Western blot. After siRNAs targeting Lin28A/B were transfected into HCC cells, the expression of Lin28A/B was detected by real-time PCR and Western blot, cell pro- liferation was assessed by MTT assay, and cell apoptosis was analyzed by FACS.RESULTS： Real-time PCR showed that the ex- pression of Lin28A/B mRNAs was up-regulated in HCC cells （P 〈 0.05; P〈 0.01）, and the up- regulation of Lin28B was more significant than that of Lin28A. Transfection of Lin28A/B siRNAs decreased the expression of Lin28A/B in HCC cells （P 〈 0.05）. MTT assay revealed that Lin28A and Lin28B siRNAs significantly inhibited HCC cell proliferation （P 〈 0.05; P 〈 0.01） and promot- ed cell apoptosis （P 〈 0.05; P 〈 0.01）. The effect of Lin28B siRNA on cell proliferation and apop- tosis was stronger than that of Lin28A siRNA （P 〈 0.05）.CONCLUSION：Both Lin28A/B take part in thetumorigenesis of HCC. Lin28B may play a majorrole in HCC tumorigenesis, while Lin28A mayplay a subordinate role.
出处
《世界华人消化杂志》
CAS
北大核心
2014年第26期3891-3897,共7页
World Chinese Journal of Digestology
基金
北华大学教育基金会永大肝病基金资助项目~~
