摘要
目的观察脂联素受体(APNR)在小鼠脑缺血模型中的表达,有助于对脂联素作用机制的研究。方法 60只健康雄性CD-1小鼠行短暂性大脑中动脉栓塞术,分别于缺血前、再灌注后1、4h、1、3及7d时处死取脑,每个时间点12只。用PT-PCR、Western blot和免疫组织化学的方法观察小鼠脑缺血再灌注后各时间点脑内APNR蛋白和mRNA表达。结果与缺血前比较,再灌注后4h、1、3、7d缺血侧脑组织中APNR1蛋白和mRNA表达量增高(P<0.05,P<0.01),缺血中心区和缺血周边区APNR1蛋白表达量相似;脑血管内皮细胞、星形胶质细胞和神经元细胞中均有APNR1的表达。各时间点小鼠缺血前后的脑组织均未见APNR2蛋白和mRNA表达。结论缺血再灌注损伤后,APNR1在缺血脑组织中表达上调,脂联素可能通过APNR1的介导发挥脑保护作用。
Objective To study the mechanism of adiponectin receptor（APNR） in protecting mouse brain by observing its expression in a mouse transient cerebral ischemia model .Methods Sixty CD-1 mice that underwent tMCAO were included in this study .Their brain tissue samples were taken before ischemia .Expression of APNR in mouse brain tissue samples taken before ischemia , and at 1 and 4 h ,and on days 3 and 7 after reperfusion was detected by RT-PCR ,Western blot and immunohistochemistry ,respectively .Results The expression level of APNR1 protein and mRNA was significantly higher in ischemic tissue at 4 h and on days 3 and 7 after reperfusion than before ischemia（P〈0 .05 ,P〈0 .01） .The expression level of APNR1 protein was similar in central is-chemic area and peripheral ischemic area .APNR1 was expressed in endothelial cells ,astrocytes and neurons .APNR2 was not expressed in brain tissue before and after ischemia .Conclusion The expression of APNR1 is up-regulated in ischemic brain tissue following ischemia-reperfusion inju-ry .Adiponectin may exert its brain protective effect via APNR1 .
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2014年第10期1086-1089,共4页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
基金
国家自然科学基金青年科学基金(81100633)
关键词
脂联素
受体
脂联素
脑缺血
再灌注损伤
adiponectin
receptors,adiponectin
brain ischemia
reperfusion injury
