摘要
目的研究存活蛋白(survivin)和基质金属蛋白酶-9(MMP-9)在卵巢上皮癌组织中的表达及生物学意义,为临床早期诊断提供依据。方法选择本院2013年1月至2014年6月卵巢上皮癌患者56例(观察组),Ⅰ+Ⅱ级26例,Ⅲ+Ⅳ级30例;高、中和低分化分别20例、19例和17例。选择良性卵巢上皮肿瘤组织42份(对照组),正常卵巢组织24份(正常组)。应用免疫组织化学法观察各组织中survivin和MMP-9蛋白的表达水平。结果 survivin蛋白主要在细胞核中表达,MMP-2蛋白主要在细胞质和(或)间质中表达;观察组卵巢上皮癌组织survivin和MMP-9蛋白吸光度(A)值分别为2.16±0.81和2.20±0.63,均高于对照组(1.42±0.76和1.46±0.34),也高于正常组0.60±0.38和0.73±0.29,差异具有统计学意义(P<0.05)。且对照组survivin和MMP-9蛋白A值高于正常组(P<0.05)。Ⅲ+Ⅳ级卵巢上皮癌组织survivin和MMP-9蛋白A值分别为2.52±0.65和2.61±0.82,均高于Ⅰ+Ⅱ级(1.26±0.33和1.34±0.56)(P<0.05)。低分化卵巢上皮癌组织survivin和MMP-9蛋白A值分别为2.70±0.62和2.80±0.71,均高于中分化组(1.59±0.57和1.72±0.54),也高于高分化组(1.04±0.28和1.15±0.32),差异具有统计学意义(P<0.05)。中分化组survivin和MMP-9蛋白A值高于高分化组(P<0.05)。结论 survivin和MMP-9蛋白与卵巢上皮癌的发生、发展具有密切关系,随着分期的增加和分化程度的降低,survivin和MMP-9蛋白的表达逐渐增高。
Objective To study the expression and biological significance of survivin and Matrix metalloproteinase-9 （MMP-9） in epithelial ovarian cancer ,to provide evidence for clinical early diagnosis .Methods Fifty-six patients with epithelial ovarian cancer were selected in the hospital from January 2013 to June in 2014 （observation group） ,26 cases of grade Ⅰ and Ⅱ ,30 cases of grade Ⅲ ＋ Ⅳ .The high ,and low differentiation were respectively 20 ,19 and 17 cases .Forty-two cases of benign epithelial ovarian tumor tissue were selected as the control group ,24 cases of normal ovarian tissues （the normal group） .The expression level of survivin and MMP-9 pro-tein was observed by immunohistochemistry .Results survivin protein was expressed mainly in the nucleus ,and MMP-2 protein was expressed mainly in cytoplasm and （or） interstitial .A value of survivin and MMP-9 protein in the observation group were 2.16 ± 0.81 and 2.20 ± 0.63 ,respectively ,which is higher than those （1.42 ± 0.76 and 1.46 ± 0.34） in the control group ,also higher than those of the normal group （ 0.60 ± 0.38 and 0.73 ± 0.29） .The difference was statistically significant （P〈 0.05） .A value of survivin and MMP-9 protein in the control group were higher than those of the normal group （P〈 0.05） .A value of survivin and MMP-9 protein Ⅲ ＋ Ⅳwas 2.52 ± 0.65 and 2.61 ± 0.82 ,respectively ,higher than those of Ⅰ ＋ Ⅱ of 1.26 ± 0.33 and 1.34 ± 0.56 （P〈0.05） .A value of survivin and MM P-9 protein in low differentiated epithelial ovarian cancer tissue were 2.70 ± 0.62 and 2.80 ± 0.71 ,respectively ,higher than those of moderately differentiation group （1.59 ± 0.57 and 1.72 ± 0.54） ,also than those in the high differentiation group （1.04 ± 0.28 and 1.15 ± 0.32） ,the difference was statistically significant （P〈 0.05） .A value of survivin and MMP-9 protein in moderately differentiation group was higher than that in the high group （P〈 0.05） .Conclusion survivin and MMP-9 p
出处
《山西医药杂志》
CAS
2014年第19期2267-2269,共3页
Shanxi Medical Journal
