摘要
通过使用改良的TCM199无血清培养系统,在96孔细胞培养板中,每孔放入一个腔前卵泡等条件下,探索添加物促卵泡激素对小鼠腔前卵泡卵母细胞体外生长发育的影响,旨在摸索一套适合小鼠腔前卵泡体外生长发育的培养体系.结果表明:随着浓度的增加,卵泡和卵母细胞的直径都不同程度的有所增长,其中400mIU/mL浓度组在卵泡培养的同期时间,卵泡和卵母细胞的增长值最大,与其他各浓度组之间存在显著性差异(P<0.05).培养到第6天时,400mIU/mL浓度组卵泡存活率最高,为66.7%.从卵泡成腔率来看,卵泡培养到第4天时,600mIU/mL浓度组卵泡成腔率最高,为35%;培养到第6天时,400mIU/mL浓度组最高,为45.2%.从卵泡发生生发泡破裂、排出第一极体来看,400mIU/mL浓度组均高于其他浓度组.结果表明,400mIU/mL浓度组卵泡和卵母细胞的增长值最大;从培养时间上看,卵泡存活率、成腔率、生发泡破裂率、排出第一极体率都最高,分别为66.7%、45.2%、16.6%、14.3%.
Based on the modified TCM199 serum-free culture system,with one preantral follicle in each hole of the 96-hole cell culture plate,the influence of additive follicle stimulating hormone （FSH） on in vitro development of preantral follicles and oocytes of the mouse was researched in order to design a suitable culture system for in vitro development of preantral follicles.The results showed that with the increase of concentration,the diameters of follicles and oocytes grew respectively and the growth of follicles and oocytes at the same period of culture was the largest at the concentration of 400mIU/mL,which were significantly different from the growth at different concentrations （P＜0.05）.On the sixth day of culture,survival rate of follicles at the concentration of 400mIU/mL was the highest （66.7 ％）.Follicular cavity rate at the concentration of 600mIU/mL on the fourth day of culture were the highest （35 ％） but the highest （45.2 ％） at the concentration of 400mIU/mL on the sixth day.Breaking rate of follicular germinal vesicles and discharge rate of the first polar body at the concentration of 400mIU/mL were both higher than those at other concentrations.In conclusion,the growth of follicles and oocytes was maximum at the concentration of 400mIU/mL,and at the different periods of culture,survival rate of follicles （66.7 ％）,follicular cavity rate （45.2％）,breaking rate of follicular germinal vesicles （16.6％）,and discharge rate of the first polar body （14.3％） were very high.
出处
《甘肃科学学报》
2014年第5期31-34,共4页
Journal of Gansu Sciences
关键词
小鼠
腔前卵泡
促卵泡激素
体外发育
Mouse
Preantral follicles
Follicle stimulating hormone
In vitro development
