摘要
本文通过对毛脉酸模内生真菌的发酵培养得到其次生代谢产物,采用HPLC法对内生真菌中蒽醌类成分进行筛选。以大黄素、大黄酸、大黄酚、大黄素甲醚、芦荟大黄素五种蒽醌类成分为参照,筛选出3株真菌含大黄素、3株真菌含大黄酸、5株真菌含芦荟大黄素,其中含量最高为683μg/g、最低为8μg/g。将各菌株所含成分与各菌株分离部位的相应成分进行比较,相对宿主植物而言各菌株均有较高的含量。本实验分析方法准确、快捷,适用于毛脉酸模内生真菌的代谢产物研究。
In this paper, the secondary metabolites of anthraqulnones were obtained by fermentation of endophytic fungi of Rumex gmelini Turcz.. HPLC was used to falter different anthraquinanes from endophytic fungi of R. gmelini. With 5 an- thraquinone compounds, including emodin, rhein, chrysophanol, physcion and aloe-emodin as reference, the endophytic fungi with the secondary metabolites of anthraqulnones were screened out,in which 3 strains were screened out to contain emodin,3 strains contained rhein, and 5 strains contained aloe-emadin. Among these fungi, the highest and lowest yield of anthraquinones was 683 p,g/g and 8 p.g/g, respectively. The strains had higher concentrations of anthraquinones be- tween each strains and its respective separated parts in R. gmelini. The developed HPLC method was accurate and fast, and it was applicable to analysis metabolites of endophytic fungi from R. gmelini.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2014年第10期1634-1637,共4页
Natural Product Research and Development
基金
国家自然科学基金项目(30970300)
哈尔滨科技创新人才研究专项基金项目(2010rfxxs031)
关键词
毛脉酸模
内生真菌
HPLC
蒽醌类次生代谢产物
Rumex gmelini Turcz.
endophytlc fungi
HPLC
anthraquinones secondary metabolites
