摘要
目的探讨glypican-3对人肺癌A549细胞增殖、凋亡和迁移的影响。方法采用glypican-3 cDNA瞬时转染A549细胞,Western blot方法检测转染前后glypican-3在A549细胞中的表达水平,分别采用MTT、流式和Transwell小室研究glypican-3 cDNA对A549细胞增殖、凋亡和迁移的作用。结果转染后glypican-3在A549细胞中表达上调,细胞增殖和迁移能力增强,细胞凋亡率降低(均P<0.05)。结论 A549细胞中glypican-3过表达促进细胞增殖和迁移,抑制细胞凋亡,可能对A549细胞的生长和存活具有重要作用。
Objective To investigate the effects of glypican-3 on the proliferation,apoptosis and migration of human lung carcinoma A549 cells. Methods Glypican-3 cDNA was transiently transfected into A549 cells,and the expression of glypican-3 was detected by Western blot. The proliferation,apoptosis and migration of transfected cells were examined with MTT,flow cytometry and Transwell assays,respectively. Results The expression of glypican-3 was up-regulated in transfected A549 cells（ P〈 0. 05）. Cell proliferation and migration were enhanced,while apoptotic rate in transfected cells was decreased（ all P〈 0. 05）. Conclusion The up-regulation of glypican-3 may promote cell proliferation and migration,and inhibit the apoptosis of human lung carcinoma A549 cells.
出处
《实用肿瘤杂志》
CAS
2014年第5期427-431,共5页
Journal of Practical Oncology
基金
国家自然科学基金项目(81101599)
