摘要
目的：优选温阳活血颗粒的提取工艺.方法：采用HPLC测定芍药苷含量,流动相乙腈-0.1％磷酸水溶液（14∶86）,检测波长230 nm;利用HPLC-ELSD测定人参皂苷Rg1,Re,Rb1含量,流动相乙腈（A）-水（B）梯度洗脱（0～ 35 min,19％A;35 ～55 min,19％ ～29％ A;55 ～70 min,29％ A;70 ～ 100 min,29％～ 40％ A）.以干膏率和有效成分（人参皂苷Rg1,Re,Rb1和芍药苷）总含量的综合评分为评价指标,通过正交试验考察加水量、提取时间和提取次数对温阳活血颗粒提取工艺的影响.结果：最佳提取工艺为加8倍量水回流提取3次,每次1.5h;干膏率依次为39.65％,人参皂苷Rg1,Re,Rb1和芍药苷提取量分别为3.49,2.79,6.64,218.60 mg.结论：优选的提取工艺科学合理、简便可行,适用于温阳活血颗粒的工业化生产.
Objective：To optimize extraction technology of Wenyang Huoxue granules.Method：HPLC was adopted to determine the content of paeoniflorin with mobile phase of acetonitrile-O.1％ phosphoric acid solution （14∶ 86） and detection wavelength at 230 nm; HPLC-ELSD was employed to determine the content of ginsenoside Rg1,Re,Rb1with mobile phase of acetonitrile （A）-water （B） for gradient elution （0-35 min,19％A; 35-55 min,19％-29％A; 55-70 min,29％ A; 70-100 min,29％-40％ A）.With yield of dry extract and total contents of paeoniflorin,ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 as comprehensive evaluation index,orthogonal design was taken for investigating effects of the amount of water,extraction times and time on extraction technology.Result：Optimal extraction conditions were as followings：reflux extracted thrice with 8 times the amount of water for 1.5 hours each time ; yield of dry extract was 39.64％,contents of ginsenoside Rg1,Re,Rb1 and paeoniflorin were 3.49,2.79,6.64,218.60 mg.Conclusion：This optimized technology is reasonable,simple and suitable for industrial production of Wenyang Huoxue granules.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第20期27-30,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
沈阳市科技计划项目(F12-155-9-00)
