摘要
目的:检测乳腺浸润性导管癌微环境中CD4及CD8阳性T淋巴细胞的表达,探讨其与乳腺癌间质微血管密度(MVD)的关系。方法:应用免疫组织化学SP法检测37例乳腺浸润性导管癌组织及10例癌旁组织中CD4、CD8及CD34的表达,并对其在癌及癌旁组织中的表达差异进行分析。采用Spearman相关性分析方法分析CD4、CD8及CD4与CD8阳性表达率的比值(CD4/CD8比值)与MVD的相关性。结果:CD4在乳腺浸润性导管癌组织中的阳性表达率为(22.63±11.53)%,低于癌旁组织[(34.09±10.41)%](P<0.05),随着乳腺癌组织学级别的升高,CD4阳性表达率逐渐降低(P<0.05)。CD8在乳腺浸润性导管癌组织中的阳性表达率为(14.27±9.03)%,高于癌旁组织[(10.51±1.88)%],但差异无统计学意义(P>0.05),随着乳腺癌组织学级别的升高,CD8阳性表达率逐渐升高(P<0.01)。在癌旁组织及乳腺浸润性导管癌组织中CD4/CD8比值分别为3.24±1.02和2.16±2.17,两者比较差异无统计学意义(P>0.05);随着乳腺癌组织学级别的升高CD4/CD8比值降低(P<0.01)。乳腺浸润性导管癌组织中的MVD值明显高于癌旁组织(P<0.05),并且随乳腺癌组织学级别的升高,MVD值呈递增趋势。相关性分析,MVD与CD4阳性表达率呈负相关关系(r=-1.000,P<0.001),与CD8阳性表达率呈正相关关系(r=0.984,P<0.001),与CD4/CD8比值呈负相关关系(r=-0.984,P<0.001)。结论:乳腺浸润性导管癌间质CD4阳性T淋巴细胞减少,CD8阳性T淋巴细胞增多,CD4/CD8比值下降,提示在乳腺癌微环境中T细胞处于免疫抑制状态。
Objective To detect the percentages of CD4＋, CD8＋ T lymphocytes in the breast invasive ductal carcinoma microenvironment,and to analyze their correlation with breast carcinoma microvessel density （MVD）. Methods The expressions of CD4,CD8 and CD34 were detected in 10 cases of adjacent normal breast tissue and 37 cases of breast invasive ductal carcinoma tissue by immunohistochemistry.The correlations between CD4,CD8, CD4/CD8 and MVD were analyzed by Spearman correlation analysis. Results The CD4 expression level （22.63%±11.53%）in breast invasive ductal carcinoma tissue was lower than that in adjacent tissue（34.09%± 10.41%）,and there was statistically significant difference （P〉0.05 ）. With the increasing of the histological grade of breast cancer,grade,the expression level of CD4 was gradually decreased（P〉0.05）.The CD8 expression level （14.27%±9.03%）in breast invasive ductal carcinoma tissue was higher than that in adjacent tissue （10.51%±1.88%）,but there was no statistically significant difference （P〈0.05）.With the increasing of the histological grade of breast cancer,the expression level of CD8 was gradually increased（P〉0.01）.The CD4/CD8 ratios in adjacent tissue group and breast invasive ductal carcinoma tissue group were 3.24±1.02 and 2.16±2.17,and there was no statistically significant difference between them （P〈0.05）.With the increasing of histological grade of breast invasive ductal carcinoma,the CD4/CD8 ratios were decreased （P〉0.01）.The MVD in breast invasive ductal carcinoma tissue was obviously higher than that in adjacent tissue,and it showed an increasing trend with the increasing of the histological grade of breast invasive ductal carcinoma（P〉0.05）.The correlation analysis showed that the MVD was negatively correlated with the CD4 expression（r=-1.000,P〉0.001）as well as CD4/CD8 ratio（r=-0.984,P〉0.001 ）, and was positively correlated with the CD8 expression level （r=0.984,P〉0.001）.Conclusion The expression of CD4 i
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2014年第5期1069-1073,I0005,I0006,共7页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅自然科学基金资助课题(20130102084JC)
吉林大学白求恩医学科研支持计划资助课题(2013101005)
吉林大学“大学生创新创业训练计划”国家级项目资助课题(2013A71249)
