摘要
以小球藻(Chlorella sorokiniana)为研究对象,考察了不同的培养基,不同的氮源,氮浓度和磷浓度对其细胞生长及营养成分的影响。结果表明,小球藻细胞生长最佳培养基为BBM培养基。不同培养基对其油脂、蛋白质和色素含量影响较小,BG-11培养基下多糖含量(44.46%)明显高于其他培养基。以甘氨酸为氮源时小球藻的生物量、油脂、蛋白质、多糖和色素含量均高于其他组。小球藻细胞生长最佳氮浓度为13.33mmol/L,缺氮情况下生物量、油脂、蛋白质和色素含量较低,但多糖含量较高,约为其他组的2倍。小球藻细胞生长最佳磷浓度为1.72mmol/L。随着磷浓度升高,小球藻生物量、油脂和蛋白质含量先增大后降低,多糖和色素含量随磷浓度升高略有降低。
The cell growth and nutritional components in Chlorella sorokiniana under different culture medium, different nitrogen sources,concentration of nitrogen and concentration of phosphorus was investigated. Results showed that the most adequate culture medium was BBM. There was no significant difference to the content of lipid, protein and pigment under different culture medium,the highest content of polysaccharose was 44.46% in the BG-11 medium. The best nitrogen source was glycine,with which the biomass,content of lipid,protein and polysaccharose were higher than other nitrogen sources. The optimum concentration of nitrogen source was 13.33mmol/L. Without any nitrogen sources,the biomass,content of lipid,protein,and pigment were least,but the polysaccharose content was highest,which was 2 folds of other groups. The optimum concentration of phosphorus was 1.72mmol/L. With increasing concentration of phosphorus,the biomass,lipid and protein content of Chlorella sorokiniana were increasing at first and then decreasing,and polysaccharides and pigment content were decreasing slightly.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第20期208-212,共5页
Science and Technology of Food Industry
基金
国家科技支撑计划项目课题(2011BAD14B01)
海南省中药现代化科技专项(ZY201327)
国家科技型中小企业技术创新基金(13C26244604892)
关键词
小球藻
细胞生长
培养基
氮源
磷
Chlorella sorokiniana
cell growth
culture medium
nitrogen sources
phosphorus
