摘要
目的 探讨白细胞介素（IL）-22在玫瑰糠疹发病机制中的作用.方法 荧光实时定量反转录（RT）-PCR检测玫瑰糠疹患者急性期、恢复期及健康对照组PBMC中IL-22mRNA的表达水平.采用酶联免疫吸附试验检测血清中IL-22的水平.结果 玫瑰糠疹患者急性期、恢复期及健康对照组PBMC中IL-22mRNA的相对表达量分别为4.15±1.36、2.89±0.91和1.91±0.60,急性期明显高于恢复期（t=3.61,P＜0.05）,恢复期高于健康对照组（f=3.84,P＜ 0.05）.玫瑰糠疹患者急性期、恢复期及健康对照组血清中IL-22水平分别为（36.87±10.63）、（21.44±7.33）和（13.22±4.26） ng/L,急性期明显高于恢复期（t=5.01,P＜0.05）,恢复期高于健康对照组（f=4.49,P＜0.05）.血清IL-22水平与玫瑰糠疹病程呈正相关（r=0.883,P＜ 0.05）.结论 IL-22在玫瑰糠疹患者外周血及血清中表达明显升高,可能在玫瑰糠疹的发病中起一定作用.
Objective To investigate the role of interleukin-22 （IL-22） in the pathogenesis of pityriasis rosea （PR）.Methods Peripheral venous blood samples were obtained from 48 patients with PR in acute stage and recovery stage and from 20 healthy human controls.Real-time fluorescence-based quantitative PCR was performed to measure the mRNA expression of IL-22 in peripheral blood mononuclear cells （PBMCs）,and enzyme-linked immunosorbent assay （ELISA） to determine the level of IL-22 in sera.Results The relative expression level of IL-22 mRNA in the patients with PR in recovery stage was significantly higher than that in the healthy controls （2.89 ±0.91 vs.1.91 ± 0.60,t =3.84,P ＜ 0.05）,but lower than that in the patients in acute stage （2.89 ± 0.91 vs.4.15 ±1.36,t =3.61,P ＜ 0.05）.Similarly,the level of serum IL-22 was significantly increased in the patients with PR in recovery stage compared with the healthy controls （（21.44 ± 7.33） vs.（13.22 ± 4.26） ng/L,t =4.49,P ＜ 0.05）,and increased in the patients with PR in acute stage compared with those in recovery stage （（36.87 ± 10.63） vs.（21.44 ± 7.33） ng/L,t =5.01,P ＜ 0.05）.The level of serum IL-22 was positively correlated with the stage of PR （r =0.883,P ＜ 0.05）.Conclusions IL-22 is overexpressed in PBMCs and sera of patients with PR,implying that IL-22 is involved in the pathogenesis of PR.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2014年第10期746-747,共2页
Chinese Journal of Dermatology
