摘要
目的:研究康艾注射液对大鼠巨噬细胞内酶活性的影响及免疫器官胸腺、脾脏形态变化。方法:雄性SD大鼠24只,随机均分为实验组与对照组(n=12)。实验组按5 ml/kg·d腹腔注射康艾注射液,对照组注射等容积生理盐水,持续注射30 d。股动脉放血处死大鼠,分别获取大鼠肺泡巨噬细胞(AM)和腹腔巨噬细胞(PM),用二辛可酸法(BCA法)测超微蛋白含量,用生化方法测定AM和PM内乳酸脱氢酶(LDH)、谷胱甘肽过氧化物酶(GSHPx)和诱导型一氧化氮合酶(iNOS)的活性,用酶联免疫吸附试验(ELISA)法测酸性磷酸酶(ACP)、超氧化物歧化酶(SOD)和琥珀酸脱氢酶(SDH)的活性。光镜下观察脾脏和胸腺的形态学改变。结果:实验组大鼠AM和PM内LDH、iNOS和GSH-Px酶活性均高于对照组(均P<0.05);ACP、SOD和SDH活性均高于对照组(均P<0.05)。光镜下,实验组脾脏动脉周围淋巴鞘增厚,淋巴细胞层次增加,淋巴小结体积增大,中央可见生发中心扩大;两组胸腺在形态学上未见明显差异。结论:康艾注射液可激活巨噬细胞,具有增强机体免疫力的作用。
Objective： To study the effects of Kangai injection on the enzyme activities of macrophages and morphology of spleen and thy- mus from rats. Methods： Twenty four male SD rats were randomly divided into two groups（ n = 12）, normal control group and experimental group. The rats in experimental group were injected with Kangai injection at the dosage of 5 ml/kg · d for 30 days peritoneally and those in con- tml group were injected with nomal saline at the same volume. The content of supermicro protein was assayed by BCA method, the activities of lactate dehydrogenase（LDH）, glutathione pemxidase（GSH-Px）, and inducible nitric oxide synthase （iNOS） from alveolar macmphages（AM） and peritoneal macmphages（PM） were detected biochemically. The activities of acid phosphatase （ACP）, superoxide dismutase（SOD） and succinate dehydrogenase（SDH）from AM and PM were detected by ELISA. The morphology of spleen and thymus were observed by light mi- crescopy. Results： The activities of LDH, GSH-Px and iNOS within AM and PM from experimental group were increased significantly compared with those of control group （ P 〈0.05）. the activities of ACP, SOD and SDH in AM and PM from experimental group were also higher than those from control group（ P 〈 0.05）. Microscopically, there was thickening of peripheral arterial lymphatic sheath, enlargement of splenic lymphoid nodules with expended germinal center in the spleen of experimental group. There was no significant difference in the mophology of thymus between the two groups. Conehsion： Kangai inieetion may improve immune function by activating macrophages.
出处
《中国应用生理学杂志》
CAS
CSCD
2014年第5期417-420,I0011,共5页
Chinese Journal of Applied Physiology
基金
浙江省教育厅资助项目(20061772)
温州市科技局资助项目(y2004A023)
