摘要
目的以阳离子脂质体介导bFGF(basic fibroblast growth factor,碱性成纤维细胞生长因子)基因,经完整圆窗膜途径转染爆震后的大鼠耳蜗,观察bFGF的表达及其对爆震性聋的防治作用。方法 SD大鼠30只,随机分为四组:空白对照组(n=6),仅接受155dB SPL脉冲噪声暴露20次;EGFP(enhanced green fluorescent protein,增强型绿色荧光蛋白)对照组(n=8),噪声暴露后即刻导入EGFP/阳离子脂质体复合物;bFGF治疗组(n=8),噪声暴露后即刻导入EGFP-bFGF/阳离子脂质体复合物;bFGF保护组(n=8),噪声暴露前3天导入EGFP-bFGF/阳离子脂质体复合物。各组动物分别于噪声暴露前及噪声暴露后1天、3天、7天、14天行ABR阈值测试。噪声暴露后14天耳蜗取材做基底膜铺片,荧光显微镜观察,并做bFGF免疫荧光染色验证bFGF的表达。结果爆震后1天,bFGF保护组ABR阈值低于空白对照组及EGFP对照组,且差异均有显著性意义(P<0.05)。爆震后3天、7天和14天,bFGF治疗组及bFGF保护组ABR阈值均低于两个对照组,且差异有显著性意义(P<0.05)。而爆震前及爆震后bFGF治疗组和bFGF保护组间的ABR阈值差异均无显著性意义(P>0.05)。术后14天,荧光显微镜直接观察及bFGF免疫荧光染色均检测到毛细胞内有bFGF的定位表达。结论将阳离子脂质体介导的bFGF基因经圆窗膜导入大鼠耳蜗能够表达,表达产生的bFGF对爆震所致的耳蜗损害有一定的保护作用,它能够减轻爆震后听。
Objective To study effects of the transduction efficiency of cationic liposome mediated EGFP-bFGF gene into the cochlea through an intact round window membrane （RWM） in rats with noise-induced hearing loss, and to assess its influence on hearing. Methods Thirty SD adult rats were divided into four groups： （1） noise exposure group： exposure to 155 dB SPL im- pulse noise 20 times only （n=6）; （2） EGFP control group： cationic liposome-pEGFP complex administered immediately after im- pulse noise exposure （n=8）; （3） bFGF rescue group： cationic liposome-pEGFP-bFGF complex administered immediately after impulse noise （n=8）; （4） bFGF protection group： cationic liposome-pEGFP-bFGF complex administered 3 clays before impulse noise （n=8）. ABR thresholds were measured in all the animals before and after blast on day 1, day 3, day 7 and day 14, respec- tively. On postoperative day 14, all the animals were sacrificed, bilateral cochleae were harvested and surface preparations of basilar membrane were studied. The expression of hFGF in hair eefls was detected by immunofluoreseent staining. Results At day 1 after blast, the ABR threshold of the bFGF protection group was lower than the noise group and EGFP control group （P 〈0.05）. At day 3, day 7 and day 14 after blast, the ABR threshold of the bFGF protection group and bFGF rescue group showed significant change compared with the two control groups （P 〈0.05）. On postoperative day 14, fluorescent microscopy showed green fluorescence in the cochlea on operated side in the prevention and rescue groups. Conclusion Cationic liposome mediated bFGF gene transferred into the cochlea through the RWM can be expressed, and can partially facilitate recovery of hear- ing loss caused by impulse noise.
出处
《中华耳科学杂志》
CSCD
北大核心
2014年第3期481-484,共4页
Chinese Journal of Otology
基金
卫生公益性行业科研专项基金(201202005)
首都临床特色应用研究专项基金(Z131107002213123)联合资助
