摘要
β-甘露聚糖酶在生物能源、饲料、食品和纺织等工业中均有着重要的应用前景。其属于半纤维素酶类,广泛存在于动植物和微生物中,微生物来源尤为广泛。随着极端微生物和极端酶的广泛研究,嗜热甘露聚糖酶因其在高温环境中具有较高酶活性和稳定性而倍受关注,并取得了较大的研究进展。本文综述了β-甘露聚糖酶的来源、分类和水解催化方式,以及嗜热甘露聚糖酶的优势和其在基因资源挖掘、重组表达以及分子改良方面的研究进展,展望了嗜热β-甘露聚糖酶未来可能的研究方向和发展前景。
β-Mannanase has important application prospect in bioenergy, feed, food, textile and other industries. β-Mannanase which belongs to hemicellulose enzymes exists in a wide variety of plants, animals and especially in microorganisms. With the extensive research of extremophiles and extreme enzymes, therrnophilic mannanases receive much concern because of their high enzyme activity and stability at high temperature. This paper reviewed the source and classification of β-mannanases and the hydrolysis pattern of thermophilic β-mannanase, as well the gene cloning, expression and molecular improvement. The research orientation and development prospects were also elucidated in this paper.
出处
《生物技术进展》
2014年第5期340-345,共6页
Current Biotechnology
基金
国家自然科学基金项目(31172235)资助
关键词
Β-甘露聚糖酶
嗜热酶
基因资源
分子改良
β-mannanases
thermophilic enzyme
genetic resources
molecular improvement
