摘要
目的通过检测妊娠期糖尿病(GDM)孕妇血清、脐血、胎盘组织中纤溶酶原激活物抑制剂-1(PAI-1)、脂联素(AP)、白介素-1(IL-1)的表达水平,探讨其与GDM发生、发展的关系。方法选择50例GDM孕妇为试验组,50例正常孕妇为对照组。采用ELISA法测定2组孕妇血清与脐血PAI-1、AP、IL-1表达水平,采用实时定量PCR测定2组胎盘组织中PAI-1、AP、IL-1表达水平。结果试验组血清、脐血、胎盘组织AP表达水平均较对照组显著降低(P<0.05),而PAI-1、IL-1表达水平均较对照组显著升高(P<0.05)。结论 AP的低表达、PAI-1与IL-1的高表达可能与GDM的发生、发展有关。
Objective To investigate the relationships ofthe levels of plasminogen activator in-hibitor-1（PAI-1）,adiponectin（AP）and interleukin-1（IL-1）expression in maternalserum,umbili-cal cord blood andplacental tissue to the occurrence and development of gestational diabetes melli-tus（GDM）.Methods The expression levels of PAI-1,AP and IL-1 in maternal serum and umbili-cal cord blood were measured by ELISA and those in placental tissue were detected by real-time PCR in 50 GDM women（experimental group）and 50 normal pregnant women（control group）.Re-sults Compared with control group,AP expression decreased but PAI-1 and IL-1 expression in-creased in maternal serum,umbilical cord blood and placental tissue in experimental group（P 〈0.05）.Conclusion The down-regulation of APexpression and the up-regulation of PAI-1and IL-1 expression may be involved inthe occurrence and development of GDM.
出处
《南昌大学学报（医学版）》
CAS
2014年第8期70-72,共3页
Journal of Nanchang University：Medical Sciences
