摘要
为阐明紫苏[Perilla frutescens(L.)Britt.]响应镉胁迫的分子机制,应用营养液加镉法,采用蛋白质组学技术分析了紫苏叶片响应镉胁迫3周的蛋白质表达差异。结果表明,镉胁迫下紫苏叶片有25个蛋白发生差异表达,其中20个蛋白质得到LC-MS/MS鉴定:光合作用相关蛋白3个,能量代谢相关蛋白11个,胁迫相关蛋白1个,蛋白质代谢相关蛋白2个,基因表达相关蛋白1个,结构蛋白1个,生物合成与解毒相关蛋白1个。在浓度为2.0 mg·kg-1、5.0 mg·kg-1、10.0mg·kg-1镉胁迫下,紫苏叶片中ATP合成酶、丝氨酸羧肽酶、植物细胞色素P450均上调表达,Rubisco大亚基、核糖体蛋白S3和肌动蛋白表达均下调。光合系统Ⅱ稳定/装配因子HCF136及胁迫反应蛋白乙酰辅酶A硫酯酶在低浓度镉(2 mg·kg-1)处理下表达上调,在高浓度镉(5 mg·kg-1,10 mg·kg-1)处理下表达下调;磷酸核酮糖激酶/尿苷激酶家族蛋白在2 mg·kg-1和5 mg·kg-1镉处理时表达上调,10 mg·kg-1镉处理时不变;逆转录转座子蛋白在10 mg·kg-1镉处理时表达下调。可见,紫苏叶片通过增强能量代谢、降低光合作用、改变蛋白代谢与基因表达和提高解毒能力,增强了镉耐性。
To elucidate the mechanism of tolerant of exposed Perilla frutescens（L.） Britt. to cadmium stress, a set of hydroponic culture experiments were set up to analyze the differential expressions of proteins in P. frutescens leaves after 3 weeks of exposure.The study used two-dimensional electrophoresis technique and added Cd2＋ to hydroponic solutions of the hydroponic culture experiments. Based on the results, 25 proteins changed in P. frutescens leaves and 20 of them were identified by LC-MS/MS analysis.The identified proteins included 3 proteins related to photosynthesis, 11 proteins related to energy metabolism, 1 protein related to stress, 2 proteins related to protein metabolism, 1 protein related to gene expression, 1 structural protein, and 1 protein related to biosynthesis and detoxication. Under Cd2＋ concentrations of 2.0 mg·kg-1, 5.0 mg·kg-1and 10.0 mg·kg-1, ATP synthase, serine carboxypeptidases and plant cytochrome P450 up-regulated in P. frutescens leaves, but oxygenase large subunit, ribosomal protein S3 and actin down-regulated in P. frutescens leaves. Furthermore, photosystem Ⅱ stability/assembly factor HCF136 and acyl-CoA thioesterase up-regulated in low Cd2＋ concentration of 2 mg·kg-1, but down-regulated in higher Cd2＋ concentrations of 5 mg·kg-1and10 mg·kg-1. Phosphoribulokinase/uridine kinase family protein up-regulated under 2 mg·kg-1and 5 mg·kg-1Cd2＋ concentrations, but no difference was detected under 10 mg·kg-1Cd2＋ concentration. Additionally, retrotransposon protein involved in gene expressiondown-regulated under 10 mg·kg- 1Cd2＋ concentration. The results indicated that P. frutescens strengthened energy metabolism,reduced photosynthesis, altered protein metabolism and gene expression, and improved detoxification under Cd2＋ stress, and thereby enhanced P. frutescens cadmium tolerance.
出处
《中国生态农业学报》
CAS
CSCD
北大核心
2014年第10期1207-1213,共7页
Chinese Journal of Eco-Agriculture
基金
国家自然科学基金项目(31070403)
福建省自然科学基金项目(2009J01056
2013J01083)
福建省教育厅基金项目(JA09084)
福建省高校服务海西建设重点项目(0B08B005)资助
关键词
紫苏
镉胁迫
镉耐性
重金属污染
蛋白质组学
Perilla frutescens（L.） Britt.
Cadmium stress
Cadmium tolerance
Heavy metal pollution
Proteomics
