摘要
目的研究磷脂酶Cε(phospholipase C epsilon,PLCε)对人骨肉瘤细胞株U2OS迁移侵袭能力的影响。方法 siRNA干扰U2OS细胞PLCε的表达,应用CCK-8实验检测PLCε对细胞增殖能力的影响,以划痕实验、Transwell小室模型实验测定细胞迁移能力改变情况,以明胶酶谱实验测定细胞分泌基质金属蛋白酶MMP2的变化。结果体外合成特异性PLCε基因siRNA可明显抑制U2OS细胞中PLCε的表达;沉默PLCε后,U2OS细胞的增殖能力无明显变化,而划痕愈合能力、迁移活力较对照组细胞明显下降,其分泌MMP2的水平也明显下降。结论沉默PLCε可致人骨肉瘤细胞U2OS的迁移和侵袭能力下降。
Aim To investigate the effects of PLCε on the invasion and migration of human osteosarcoma cancer cells U2OS. Methods RNA interference （ RNAi） was used to inhibit PLCεexpression, and the proliferation of cancer cells was measured by CCK-8 assay. The migration of the cells was measured by scratch wound healing assay and migration chamber as-say. Gelatin zymography was performed to measure the MMP2 activities in U2OS cells. Results PLCε ex-pression was suppressed by siRNA. CCK-8 assay showed that PLCε had no effect on the proliferation of cancer cells. PLCε knockdown inhibited cell invasion and activities of MMP2 . Conclusion PLCε knock-down can inhibit the migration and invasion of human osteosarcoma cancer cells U2OS.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2014年第10期1388-1392,共5页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81372172)
教育部重点课题项目(No 212062)
