摘要
目的 探讨NO在镉致急性肝损伤中的作用以及慈菇的干预效果。方法 30只雄性SD大鼠随机分为 5组 ,每组 6只 ,分别用 2 5 %、5 0 %、75 %的慈菇 (2ml/ 10 0 g体重 )及等体积蒸馏水灌胃 10天 ,未次灌胃 2 4h后 ,腹腔注射Cd Cl2 (2 0 μmol/L/kg体重 )或蒸馏水 ,染毒 2 4h后 ,观察血清NO含量、GPT、LDH活力及肝组织NOS活力、MDA合量。结果 CdCl2 组与阴性对照组比较 ,血清LDH、GPT活力 ,肝组织MDA合量显著升高 (P <0 0 5 ) ,中、高浓度慈菇干预组相比CdCl2 组LDH、GPT活力及MDA含量显著降低 (P <0 0 5 ) ;血清中NO含量及肝组织NOS活性各组间无明显差异 (P >0 0 5 ) ,不同浓度慈菇对肝组织NOS活性及血清NO浓度的降低具有剂量反应关系 (r分别为 - 0 95 96、-0 995 6 ,P <0 0 5 )。结论 NO可能参与镉所致肝脏的急性过氧化损伤 。
Objective To research the effect of nitric oxide in the process of cadmium-induced acute liver injury and intervention with Sagittaria Sagittifolia.Methods Thirty male Sprague-Dawley rats were randomly assigned into six groups,each of six.The rats were pretreated intragastrically with 25%,50%, 75% Sagittaria Sagittfolia extract(2ml/100g) or distilled water once daily for ten days.Twenty-four hours after the last dosing,animals were administered with CdCl 2 (20μmol/L/kg) intraperitoneally,control animals were given distilled water only.Twenty-four hours after the intoxication,the serum LDH,GPT activity and NO concentration,liver MDA content and nitric oxide synthase (NOS) activity were measured in each group above.Results Compared to the control,cadmium could increase the serum LDH,GPT activity and liver MDA content( P <0.05); Treatment of rats with 50% and 75% Sagittaria Sagittifolia extract could decrease the serum LDH,GPT activity and liver MDA content( P <0.05);Sagittaria Sagittifolia extract inhabited liver NOS activity and decrease serum NO concentration in dose-dependent manner ( r=-0.959 6,-0.995 6,P <0.05 ) also.Conclusion It is suggested that NO seems to be involved in cadmium-induced acute peroxidative liver injury on rats and the intervention effect of Sagittaria Sagittifolia on rats.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2002年第8期907-908,共2页
Chinese Journal of Public Health
关键词
一氧化氮
镉
急性肝损伤
慈菇
干预效果
nitric oxide
cadmium
acute liver injury
sagittaria sagittifolia
