摘要
目的：观察中等强度持续训练后骨骼肌线粒体抗氧化能力和ATP酶活性的变化。方法 SD大鼠24只，8只为正常对照组（A组），16只采用递增负荷训练建立中等强度持续运动跑台训练模型，又均分为运动2周及6周组（B及C组）。B及C组大鼠按Bradford等的方法分别训练2和6周，每周训练6 d。训练结束后大鼠处死，用差速离心法提取腓肠肌线粒体，紫外-可见分光光度计检测锰超氧化物歧化酶（Mn-SOD）活性；脂质过氧化产物丙二醛（MDA）含量采用TBA（硫代巴比妥酸）法测定；Na＋K＋-ATP酶和Ca2＋Mg2＋-ATP酶活性采用酶促反应定磷法测定。结果B组的骨骼肌线粒体Mn-SOD水平与A组相比差异无统计学意义，P＆gt;0.05，而MDA含量显著高于对照组，P〈0.05。C组的Mn-SOD水平显著高于A组， P〈0.05，而MDA含量显著低于A组，P〈0.05。B组Na＋K＋-ATP酶和Ca2＋Mg2＋-ATP酶活性均显著低于A组，均P〈0.05，而C组的Na＋K＋-ATP酶和Ca2＋Mg2＋-ATP酶活性均显著高于A组，均P〈0.05。结论中等强度持续训练一定时间后可以增加骨骼肌线粒体Mn-SOD的活性，降低脂质过氧化水平，减少自由基的损害作用，提高ATP酶活性，改善线粒体能量代谢水平。
Objective To investigate the effects of moderate-intensity continuous training on the mitochondrial antioxidant capacity and activity of ATPase in skeletal muscle so as to provede objective basis for scientific exercise training. Methods Twenty-four SD rats were randomly divided into 2 groups：normal control group （Group A, n=8） and experimental group （n=16）. Those rats in the experimental group underwent treadmill exercise at moderate intensity according to the Bredford＆#39;s model. They were further randomly divided into 2 equal groups to be trained 6 days a week for 2 weeks （Group B） or 6 weeks （Group C）. At the end of exercise training all rats were killed with their gastrocnemius muscle obtained and mitochondria therein were extracted by differential centrifugation. Ultraviolet-visible light spectrophotometer was used to examine the activities of manganese superoxide dismutase （Mn SOD） and copper zinc superoxide dismutase （CuZn SOD） . Thiobarbituric acid method was used to detect the content of malondialdehyde （MDA）, a lipid peroxidation product. Enzyme reaction phosphorus measurement was used to detect the acxtivities of Na＋K＋-ATPase and Ca2＋Mg2＋-ATPase. Results There was no significant difference in the content of Mn-SOD between Group A and Group B ,P＆gt;0.05, whereas the content of MDA of Group C was significantly higher than that of Group A ,P〈0.05. The Mn-SOD level of Group C was significantly higher than that of Group A,P〈0.05, however, the content of MDA of Group C was signiuficantly lower than that of Groupo A ,P〈0.05. The activities of Na＋K＋-ATPase and Ca2＋Mg2＋-ATPase of Group B were both significantly lower than those of Group A,both P〈0.05, however, those of Group C were both significantly higher than that of Group A ,P 〈0.05. Conclusion A certain period of moderate-intensity continuous training increases the activity of Mn-SOD, decreases the level of lipid peroxidation, reduces the injury of free radical, enhances the activity of ATPase,
出处
《中国急救复苏与灾害医学杂志》
2014年第9期837-840,共4页
China Journal of Emergency Resuscitation and Disaster Medicine
基金
武警后勤学院重点科研项目（WKH2009202）
武警后勤学院面上项目（WHM201204）
关键词
跑台运动
骨骼肌
线粒体
超氧化物歧化酶
三磷酸腺苷酶
Treadmill exercise
Skeletal muscle
Mitochondria
Superoxide dismutase
Adenosine triphosphatase
