摘要
采用液相色谱-串联质谱（LC-MS/MS）法检测鸡蛋中金刚烷胺,比较不同类型色谱柱、提取溶液（甲醇-1%三氯乙酸水溶液与甲醇/氨-氯化铵缓冲液）和净化方式（正己烷分配去脂、正己烷/二氯甲烷反萃取及Oasis MCX固相萃取柱净化）。结果显示,用甲醇-1%三氯乙酸水溶液提取,正己烷分配去脂,经Agilent XDB-C18（150 mm×2.1 mm,3.5 m）色谱柱分离,LC-MS/MS检测效果最好。本方法检出限（LOD）为2μg/kg,线性范围在1~1 000μg/L之间,回收率88.0%~99.6%。方法操作简便,实用性强,精密度好,为禽产品的质量安全检测与监管提供方法依据。
An analytical method was developed to determine amantadine in fresh eggs by LC-MS/MS. Different chromatographic column, two extraction systems, which were methanol：l% trichloroacetic acid aqueous solution and methanol / ammonia-ammonium chloride buffer, and three purification systems, which were assigned fat by N-hexane, N-hexane / dichloromethane anti-extraction and Oasis MCX solid phase extraction column purification were considered. The optimum condition was achieved by extracted with a mixed solvent of methanol-l% trichloroacetic acid, removing lipids with N- hexane, and separating by Agilent XDB-C18 （150mm×2.1 mm,3.5 m）. The linear range wasl-1 000 μg/kg, and the limit of detection was 2 μg/kg. The recoveries ranged from 88.0%-99.6%. The method provides scientific basis for the supervision of poultry eggs safety with simplified operation, good practicability and higher sensitivity.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2014年第9期190-194,共5页
Journal of Chinese Institute Of Food Science and Technology
基金
2014年国家畜禽产品质量安全风险评估项目(GJFP2014007)
“十二五”国家科技支撑计划课题(2012BAD28B03)
现代农业产业技术体系专项资金资助(CARS-42)
