摘要
目的:通过组织学研究评价羟基磷灰石/凝胶(HAP-GEL)支架复合成骨细胞在兔颅骨缺损中的修复效果。方法:将HAP-GEL复合物与成骨细胞联合培养,制备兔颅顶骨骨缺损模型,并设置空白对照。于术后4,8,12周通过组织学观察分析骨缺损修复情况。结果:组织学观察,随着时间的推移,HAP-GEL支架组复合成骨细胞逐步出现降解并有新生骨小梁产生,材料密度降低。结论:HAP-GEL支架复合成骨细胞在兔颅骨缺损中可取得较好的修复效果。
Objective：To investigate the ability of HAP-GEL/osteoblasts scaffolds to repair cranial bone defect by his-tological study. Method：HAP-GEL and osteoblasts were co-culture.The rabbits cranial parietal bone defect models were prepared.HAP-GEL scaffolds and osteoblasts,HAP-GEL scaffolds and blank defect were implanted into the cranial bone de-fects area respectively. After 4,8,12 weeks,the results were evaluated by histological examination. Result：Histological ob-servation showed HAP-GEL/osteoblasts materials had appeared degradation and the formation of new trabecular bone ,the density of the material decreased gradualy. Conclusion：The engineering bone scaffold constructed by HAP-GEL scaffolds and osteoblasts can be applied to the repair of bone defect.
出处
《临床口腔医学杂志》
2014年第9期519-521,共3页
Journal of Clinical Stomatology
基金
山东省自然科学基金(ZR2010HL046)
山东省卫生厅科技发展计划项目(2011HZ018)
