摘要
目的研究不同形式跳跃运动对大鼠胫骨白介素6(IL-6)和骨代谢相关分子护骨素(OPG)、核因子-κB受体激活因子配体(RANKL)基因表达的影响,探讨过度跳跃运动所致的骨质破坏发生的可能机制。方法 24只8周龄雄性SD大鼠,随机平均分为对照组、跳跃组和跳跃负重组。跳跃组进行递增负荷跳跃训练,负重跳跃组按5%自身体质量负重,训练计划同跳跃组,对照组不作处理,实验6周后完成取材测试。采用比色法检测血清碱性磷酸酶(ALP)和抗酒石酸酸性磷酸酶(TRACP)浓度,取一侧胫骨制作HE石蜡切片,另一侧用实时定量PCR检测IL-6、OPG和RANKL的mRNA表达。结果与对照组和跳跃组相比,跳跃负重组骨胶原纤维和骨膜排列紊乱,骨皮质出现较大程度破坏,骨质中空腔增多。跳跃负重组血清ALP显著高于跳跃组和对照组(P<0.05)。跳跃负重组和跳跃组血清TRACP显著高于对照组(P<0.05)。大鼠经过2种形式跳跃运动6周后,胫骨组织IL-6 mRNA表达均显著高于对照组(P<0.01)。负重跳跃组IL-6 mRNA表达显著高于普通跳跃组(P<0.01)。与对照组比较,跳跃组和跳跃负重组OPG mRNA表达显著升高,其中跳跃组表达量最高。跳跃负重组RANKL mRNA表达显著高于跳跃组和对照组(P<0.01)。结论 IL-6和RANKL在负重跳跃运动大鼠胫骨中升高,参与以骨高转换率为特征的骨质破坏过程。
Objective To investigate the effect of different types of jumps on the expressions of interleukin 6（ IL-6）,osteoprotegerin（ OPG）,ligand of receptor activator of NF-κB（ RANKL） genes related to bone metabolism in rat tibia,and explore the molecular mechanism underlying bone destruction associated with over-loading jumping. Methods Twenty-four male SD rats were randomly assigned to control group（ C）,jump group（ J） and loading jump group（ JL）,8 rats in each group. J group participated in incremental load training. The rats in JL group carried loads that were 5% of their body mass.Same training plan was used in J and JL groups,while control group was not treated. The experiment was completed after6 weeks. The serum samples were collected and assayed for the contents of alkaline phosphatase（ ALP） and tartrate resistant acid phosphatase（ TRACP）. One-side tibia was used for paraffin section and HE staining,and the other side was used for real time quantitative PCR（ qRT-PCR） to detect the expressions of IL-6,OPG and RANKL mRNA. Results Compared with control group and J group,the collagen fibers in periost was more disordered in JL group. We further observed a more serious damage of the cortical bone and increased cavities in JL group. The serum ALP levels in JL group were significantly higher than those in control and J groups（ P〈0. 05）. The serum TRACP levels in JL and J groups were significantly higher compared to control group（ P〈 0. 05）. Furthermore,there was a significantly higher expression level of IL-6 in both jump groups（ P〈0. 01）,while the JL group had a higher IL-6 expression compared with J group（ P〈0.01）. There was a higher expression of OPG mRNA in JL and J groups（ P〉0. 05）. The highest expression level of OPG was observed in J group. The expression level of RANKL was significantly higher in JL group compared with control and J groups（ P 〈0. 01）. Conclusion After loading jump for 6 weeks,the expressions of IL-6 and RANKL were markedly e
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2014年第9期909-912,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
江苏省普通高校研究生科研创新计划项目(CXZZ13_0782)
