摘要
目的：了解浙江西天目山家畜和啮齿动物中流行的立克次体无形体属种型，分析其16S rRNA基因变异。方法从动物肝脾和血液中提取DNA，PCR扩增无形体属16S rRNA片段；测序，采用MEGA5．0软件进行序列比对和系统发生分析。结果129只动物中，1只牛、8只羊、8只啮齿动物检出无形体属16S rRNA 片段，序列比对和系统发生分析表明，检出的无形体属16S rRNA包括嗜吞噬细胞无形体、边缘无形体、中央无形体和牛无形体，还检测到变异较大的16 S rRNA基因。结论西天目山动物中存在无形体属立克次体感染，其中啮齿动物中发现的变异株可能为一种与牛无形体紧密相关的新种型。
Objective To identify Anaplasma species circulating among livestock and rodents from Xitianmu Mountain area in Zhejiang province , Southeastern China and to analyze variations regarding to their 16S rRNA gene.Methods Samples of spleen, liver and blood were collected to extract DNAs .The 16S rRNA gene fragments of Anaplasma species were amplified by using a nested PCR and then sequenced .Ho-mology analysis was conducted by using BLAST program .The multiple sequence alignment and phylogenetic analyses comparing with the sequences of other Anaplasma species in GenBank were conducted by using MEGA 5.0 software.Results The 16S rRNA gene fragments of Anaplasma were detected in 1 cattle, 8 goats, 5 Rattus confucianus, 1 Apodemus agrarius, 1 Berylmys bowersi and 1 squirrel out of 129 animals. The natural infection rate of Anaplasma was 13.2% in animals from Xitianmu Mountain area in Zhejiang . The alignment and phylogenetic analyses indicated that there were at least four Anaplasma species prevalent in livestock and rodents from Xitianmu Mountain area , including Anaplasma phagocytophilum, Anaplasma marginale, Anaplasma centrale and Anaplasma bovis.Moreover, there was a variant that obviously differed from Anaplasmma bovis and other Anaplasma sp.in GenBank.Conclusion The Anaplasma infection was detected among livestock and rodents from Xitianmu Mountain area in Zhejiang province .A newly discovered variant in rodents was likely to be a novel species .More close attention should be paid to Anaplasma infec-tion among human in Xitianmu Mountain area .
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2014年第8期589-593,共5页
Chinese Journal of Microbiology and Immunology
基金
中美新发和再发传染病合作项目（1U2GGH000018-01）
浙江省科技计划项目（2004C33001）
浙江省医药卫生科技项目（2009A045）
关键词
无形体
动物感染
基因变异
Anaplasma
Animal infection
Gene variation
