摘要
从患病杂交鳢[斑鳢（Channa maculata）♀×乌鳢（C.argus）♂]体内分离到2株致病菌（ZS201364-1和ZS201364-2）,通过对其生化特性与16S rRNA基因序列进行分析,确定为迟缓爱德华菌（Edwardsiella tarda）。该致病菌兼性厌氧,为革兰氏阴性短杆菌,能运动,能发酵葡萄糖、麦芽糖。吲哚试验和MR试验均为阳性。2株致病菌对杂交鳢的半致死剂量（LD50）分别为7.1×105cfu·g^-1和5.6×105cfu·g^-1。ZS201364-1最适生长温度为25～35℃、pH为7～8、氯化钠（NaCl）质量分数为1%,对头孢噻肟、环丙沙星、诺氟沙星等抗生素高度敏感。
Two pathogenic bacteria （ZS201364-1, ZS201364-2） isolated from diseased hybrid snakeheads （Channa maculata♀× C. argus ♂） were identified to be Edwardsiella tarda by analyses of biochemical features and 16S rDNA sequence. The anaerobic, gram negative, bacilliform and motile isolates utilized glucoses and maltose as energy. Both indole and M.R. tests proved positive. The LD50 s of the isolates to healthy hybrid snakeheads were 7. 1 × 10^5 cfu·g^-1 and 5.6 × 105 cfu·g^-1, respectively. The optimal growth conditions of strain ZS201364-1, which was highly susceptible to cefotaxime, ciprofloxacin and norfloxacin, were 25 - 35 ℃ pH 7 ～ 8 and 1% NaCl.
出处
《南方水产科学》
CAS
CSCD
北大核心
2014年第5期1-7,共7页
South China Fisheries Science
基金
佛山科学技术学院校级科研项目
广东省教育部产学研项目(2010b090400255)
关键词
杂交鳢
迟缓爱德华菌
分离
鉴定
hybrid snakehead
Edwardsiella tarda
isolation
identification
