摘要
目的:探讨参松养心胶囊(SSYX)对糖尿病(DM)大鼠心室电生理特性与结构功能变化的影响。方法:雄性SD大鼠45只,随机分为对照组(CTL组,n=15)、DM组(n=15)和SSYX组(n=15)。DM组和SSYX组采用一次性腹腔注射链脲佐菌素(60 mg/kg)制备糖尿病模型,CTL组则给予腹腔注射生理盐水(1 mL/kg),72 h后检测血糖评价造模结果,将造模成功的动物纳入本研究。SSYX组连续6周给予SSYX灌胃(1 g·kg-1·d-1)治疗,DM组和CTL组则给予生理盐水灌胃(2 mL·kg-1·d-1)。动物完成药物治疗后,采用超声心动图检测心功能并记录II导联体表心电图;采用放射免疫法检测血浆的内皮素1(ET-1)水平;随后在整体心脏Langendorff灌流条件下行离体电生理研究,分别记录和测量左室前游离壁(LAF)心外膜单相动作电位(MAP)和有效不应期(VERP),同时给予Burst快速电刺激用以进行室性心律失常(VA)的诱发;采用Masson染色对3组动物心肌纤维化程度进行评价。结果:与CTL组相比,DM组心功能下降(P<0.01),而QT间期、VERP、动作电位时程、VA诱发率、心肌纤维化程度和血浆ET-1水平均增加(均P<0.01);与DM组相比,SSYX组的心功能得到改善(均P<0.01),且QT间期、VERP、动作电位时程、VA诱发率、心肌纤维化程度和血浆ET-1水平均降低(均P<0.05)。结论:SSYX具有减轻DM大鼠心室电重构和结构重构及改善心功能的作用。
[ABSTRACT]AIM：TodeterminetheeffectsofShensongyangxincapsule（SSYX）ontheventricularelectrical properties, structural remodeling and cardiac function in the rats with diabetes mellitus （DM）.METHODS：Male SD rats （n=45） were randomly divided into control group （n=15）, DM group （n=15） and SSYX group （n=15）.The rats in DM group and SSYX group were injected with streptozotocin （60 mg/kg, ip）, while the rats in control group were given normal saline （1 mL/kg, ip）.The blood samples were collected 72 h after treatment for determining the blood glucose lev-els in DM group and SSYX group .The model rats in SSYX group were administered with SSYX （1 g＆#183; kg-1 ＆#183; d-1 , ig） for 6 weeks, while the other rats received normal saline （2 mL＆#183; kg-1＆#183; d-1, ig）.The echocardiography was used to assess the cardiac function , and the lead II electrocardiogram was also recorded in all the animals .The radioimmunoassay and Masson trichrome staining were used to measure the plasma levels of endothelin -1 （ET-1） and the collagen deposition in the ventricles, respectively.A whole Langendorff-perfused heart model was used to conduct the electrophysiologic study .The monophasic action potential （ MAP） and the ventricular effective refractory period （ VERP） were recorded in the left anteri-or free wall （ LAF） , and the burst pacing was used to induce ventricular arrhythmia （ VA） .RESULTS： Compared with control group, the VERP, action potential duration （APD）, QT interval, incidence of VA, degree of myocardial fibrosis and plasma level of ET-1 were increased , while the cardiac function was declined in DM group .Compared with DM group , the VERP, APD, QT interval, incidence of VA, degree of myocardial fibrosis and plasma level of ET-1 were all de-creased, while the cardiac function was improved in SSYX group .CONCLUSION： SSYX attenuates the electrical and structural remodeling and improves the cardiac function in DM rats .
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2014年第8期1439-1444,共6页
Chinese Journal of Pathophysiology
基金
湖北省恩施州科技局指导项目(恩州科业[2013]15号)
关键词
参松养心胶囊
糖尿病
电重构
结构重构
心功能
Shensongyangxin capsule
Diabetes mellitus
Electrical remodeling
Structural remodeling
Car-diac function
