摘要
为了研究钼对雌性小鼠骨骼形态结构的影响,选择了40只健康雌性昆明系小鼠随机分为4组,每组10只。通过在饮水中添加不同剂量的钼,建立雌性小鼠钼暴露模型:对照组、钼100组、钼200组和钼400组。在试验处理第120天时采集小鼠股骨,测量股骨长度和中点直径后制作石蜡切片,苏木素-伊红染色,用显微镜观察其形态结构的变化。试验结果显示:低剂量的钼可促进小鼠体质量的增长和骨骼的生长发育。但高剂量的钼则使股骨长度和直径显著减少(P<0.01),骨小梁数目减少、宽度变窄,骨皮质厚度增加等,并使小鼠骨骼细胞数目显著降低(P<0.01),骨骺线中分裂期细胞百分比显著下降(P<0.01)。高钼对雌性小鼠骨骼形态结构具有一定的毒性作用。
In order to investigate the effect of molybdenum on the bone morphology in female mice,40 healthy female Kunming mice were randomly divided into 4 groups of 10. Different doses of molybdenum were added into drinking water to establish the molybdenum exposed female mice models such as the control group,the Mo 100 group,the Mo 200 group,the Mo 400 group. On the 120 th day,the femurs of mice in each group were collected and the length and midpoint diameter were measured,then made into paraffin sections,hematoxylin-eosin staining(HE stained). The morphological change of the structure was observed by using microscope. The results show that the low doses of molybdenum can promote the growth of body weight and the development of bone. The high doses of molybdenum can significantly decrease the length and diameter of femurs( P < 0. 01),reduce the number of trabecular bone,narrow the diameter,and increase the cortical thickness of femur. At the same time,the number of the femur cells and the percentage of dividing cells in epiphyseal line are decreased significantly(P <0.01).High doses of molybdenum have a certain toxic effects on bone morphology in female mice.
出处
《河南科技大学学报（自然科学版）》
CAS
北大核心
2014年第6期71-74,79,共5页
Journal of Henan University of Science And Technology:Natural Science
基金
国家自然科学基金项目(31140003)
河南省教育厅自然科学研究基金项目(2010B230002)
河南科技大学博士启动基金项目(09001368)
河南科技大学大学生研究训练计划(SRTP)项目(2011190
2010088)
关键词
钼
雌性小鼠
骨骼
形态结构
molybdenum
female mice
bone
morphology
