摘要
目的:探讨胃肠道间质瘤(GIST)中胰岛素样生长因子Ⅱm RNA结合蛋白(IMP3)的表达及意义。方法:运用免疫组化SP法观察IMP3在115例GIST患者中的表达情况并分析其表达与不同临床病理参数和Ki67增殖指数之间的关系;随机选取IMP3不同程度阳性表达病例20例进行IMP3 m RNA原位杂交检测。结果:IMP3在GIST中阳性表达率为56.2%(65/115);IMP3在不同性别、年龄、肿瘤原发部位和组织学类型中的表达差异均无统计学意义(P>0.05),而在是否有局部侵犯、坏死和转移中表达差异均有统计学意义(P<0.05);随着肿瘤直径增大、细胞异型性增加、核分裂像增多及危险度增高,IMP3的阳性表达率增加,各组间比较差异有统计学意义(P<0.001);IMP3表达与Ki67指数增高呈正相关关系(r=0.348,P<0.001);3例复发病例均表达IMP3。结论:IMP3在GIST中表达与肿瘤的侵袭及危险度相关,该基因可能参与了GIST的侵袭与进展,可作为GIST预后判断的参考指标。
Objective To investigate the expression and significance of Insulin like growth factor-II mRNA binding protein3 （IMP3） in gastrointestinal stromal tumors （GIST）. Methods One hundred and fifteen cases of GIST were subjected to immunohistochemical staining for IMP3 （SP method） and ki67 （iVisionTM method ）. The relationship between IMP3 expression with clinicopathologic parameters and Ki67 proliferation indexes were analyzed. In situ hybridization detection of IMP3 mRNA was performed in 20 cases of GIST positive. Results The expression rate of IMP3 was 56.2% （65/115） in GIST positive. A significant correlation can be found between IMP3 expression and tumor of infiltration, coagulative necrosis, metastasis, nuclear atypia, diameter, mitotic count, risk grade of NIH （P 〈 0.05）, while there was no significant correlation between IMP3 and gender, age, primary location, categories of cell type （P 〉 0.05）. There was positive correlation of IMP3 expression and Ki67 labeling indexes by Spearman analysis. Positive expression with IMP3 can be observed in 3 cases of recurrence.ConclusionsThe expression of IMP3 is associated with poor prognosis in GIST. It may play important role in invasive and development and may be an important factor of prognosis in GIST.
出处
《实用医学杂志》
CAS
北大核心
2014年第19期3092-3095,共4页
The Journal of Practical Medicine
基金
贵州省卫生厅项目[编号:黔卫发(2012)84号]
