摘要
为了探讨提高小麦花药离体培养中绿苗诱导率的途径,本研究将66份小麦杂交组合材料分成花培组合、常规组合、复交组合3种不同的基因型组合,将其播种期推迟24d,在小麦抽穗期取其花药进行室内愈伤组织诱导和绿苗分化培养。结果表明,经过晚播处理的小麦花培组合、常规组合、复交组合的绿苗诱导率分别为2.6%、1.5%、1.2%,分别比对照(正常播种期)提高1.8、1.0、0.6个百分点;愈伤诱导率分别为10.7%、9.1%、7.7%,分别比对照提高3.8、-2.8、-5.4个百分点。
In order to explore the method to increase the induction rate of green plantlets in wheat anther culture,6 6 hybridization combinations were divided into three different genetype combinations：anther cultural combination,conventional breeding combination and composite combination.The planting time of them was delayed by 24 d.The callus induction and green plantlet differentiation were conducted during the heading stage.The results showed that the induction rate of green plantlets in late planting condition was 2.6%,1.5% and 1.2%, respectively,in the anther cultural combination,conventional breeding combination and composite combination,which was 1.8,1.0,0.6 percentage points higher than that of CK（normal sowing date）,respectively.The callus induction rate of three genetype combinations in late planting condition was 10.7%,9.1%,7.7%,respectively,which was 3.8,-2.8,-5.4 percentage points higher than that of CK respectively.
出处
《河南农业科学》
CSCD
北大核心
2014年第9期24-27,37,共5页
Journal of Henan Agricultural Sciences
基金
河南省科技创新杰出人才基金项目(134200510006)
关键词
晚播
小麦
花药培养
愈伤组织诱导
绿苗分化培养
绿苗诱导率
late planting
wheat
anther culture
callus induction
green plantlet differentiation
green plantlet induction
