摘要
对36个引自加拿大的豌豆品种(系)进行抗白粉病表型和标记基因型鉴定,明确了豌豆品种Cooper和Tara白粉病抗性等位基因。苗期接种了2个不同地理来源的豌豆白粉病菌分离物,32个品种(系)对2个分离物均表现为免疫;品系MP1818-2对云南白粉菌分离物EPYN免疫,但对北京分离物EPBJ感病;其余3个品种对2个分离物均感病。4个与豌豆抗白粉病基因er1连锁的SCAR标记将36个豌豆品种(系)区分为5个标记基因型。与野生型PsMLO1基因序列比较发现,豌豆品种Cooper和Tara的PsMLO1候选基因均在680 bp处发生C变G的单核苷酸突变。
The phenotype and genotype of 36 pea cultivars or lines introduced from Canada were identified for their resistance to powdery mildew. The allele of powdery mildew resistance gene er1 locus in cultivars Cooper and Tara were investigated. All tested cultivars or lines were inoculated by two powdery mildew isolates from different geographical origins in seedling stage,respectively. Thirty-two cultivars or lines were immune to the two isolates,line MP1818-2 was immune to isolate EPYN and susceptible to isolate EPBJ,whereas 3 cultivars were susceptible to the two isolates. Five marker-genotypes were identified in the 36 cultivars or lines by 4 SCAR markers linked to er1. Sequence analysis revealed that the candidate gene PsMLO1 both in Cooper and Tara contained a single nucleotide mutation which G replaced C in PsMLO1 gene at 680 bp site.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2014年第5期1028-1033,共6页
Journal of Plant Genetic Resources
基金
国家现代农业产业技术体系建设专项(CARS-09)
作物种质资源保护子项目(NB2010-2130135-25-14)
国家自然科学基金(31160304)
中国农业科学院科技创新工程项目
关键词
豌豆
白粉病
抗病性
标记基因型
er1
Pisum sativum
powdery mildew
resistance
marker genotype
er1
